Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,301	G→A	P106P (CCG→CCA)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,301	0	G	A	100.0%	31.5 / NA	13	P106P (CCG→CCA)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

CGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCCTTGGCCCGACCGCCTTAGGTGAAGGGTTGGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGC  >  NC_000913/766090‑766533                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         cgcgcTTTACCCGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCCGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCCTTGGCCCGCCCGCCTTAGGTGAAGGGTTGGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCAAACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGAt                                                                                                                                                                                                   >  1:37897/1‑251 (MQ=255)                                                                      ccAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCCTTGGCCCGACCGCCTTAGGTGAAGGGTTGGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCtn                                                                                                                              <  1:157352/251‑2 (MQ=255)                                                                                                         cGTGAAAGCCTGGGCCCGACCGCCTTAGGGGAAGGGTTGGCTGTGCCGCATGGCAAAACGTCTGCGGTAAAAGAAGCGGCGGTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGcgcg                                                                                           <  1:97163/251‑1 (MQ=255)                                                                                                                                ctggggTGAAGGGTGGGCTGTGCCGGACGGCTAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTGGCGACACCCAGGGAGCCACGTCGGTGGGAAGGCGGTGATGGCCCGGAAGCAGTTGATTTAg                                                                                                                                                                                               <  1:126374/124‑1 (MQ=255)                                                                                                                                    ggTGAAGGGTTGGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGAGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCtgatg                                                                 >  1:226556/1‑250 (MQ=255)                                                                                                                                                gCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCGCAGACAGCGAGCCACTTCAGTGGGAAGGCGTTGATAGTTCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCg                                                                                                                                                                                 <  1:209098/126‑1 (MQ=255)                                                                                                                                                gCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCggg                                                                                                                                                             >  1:1822/1‑146 (MQ=255)                                                                                                                                                           tGGCAAAACGGCTGCGGTAAAGGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGcn                                                                                                                                                                                                                          <  1:66392/74‑2 (MQ=255)                                                                                                                                                                     nctgcGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                               >  1:152543/2‑187 (MQ=255)                                                                                                                                                                     gctgcGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGTAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGc                                 <  1:104470/249‑1 (MQ=255)                                                                                                                                                                       ggggctcttccggtctgcgTTTGCGGTCGCCACACTCACCGAGCCACTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACga                             <  1:55943/235‑2 (MQ=255)                                                                                                                                                                                       gcgTTTGCGGTCGCCACACTCAGCGANCCACTTNNGTNGGAAGGCGTTGATGGCCCGNAANNNGNNNANTTAGTNGTNNTGCTGGCGANTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCANGCGCCTTGCNNATGATGAGATTCGGGCGCGTANACANNCNNCNacgncg                                                                        >  2:55943/1‑192 (MQ=255)                                                                                                                                                                                                  cGCCACACTCAGCGAGCCACTTCAGTGGGAAGGCGTTGCTGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGCAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACgcgc  >  1:186815/1‑251 (MQ=255)                                                                                                                                                                                                      nnnnnnnnnnaGNNNNNTCANTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCggg                                                                                               <  2:152543/144‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         CGCGCTTTACCAGCCGTGAAGAGGCCATCCACGCGTTGACTCAACGTCTTGCTGCTCTGGGGAAAATTTCCAGTACTGAGCAATTTCTGGAAGAAGTGTATCGCCGTGAAAGCCTTGGCCCGACCGCCTTAGGTGAAGGGTTGGCTGTGCCGCATGGCAAAACTGCTGCGGTAAAAGAAGCGGCGTTTGCGGTCGCCACACTCAGCGAGCCGCTTCAGTGGGAAGGCGTTGATGGCCCGGAAGCAGTTGATTTAGTGGTGCTGCTGGCGATTCCCCCCAATGAAGCGGGTACTACGCATATGCAACTGCTGACAGCGCTGACCACGCGCCTTGCGGATGATGAGATTCGGGCGCGTATACAGTCGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGC  >  NC_000913/766090‑766533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑