Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	14.5 / NA	8	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/4);  total (4/4)

AGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCC  >  NC_000913/1337154‑1337545                                                                                                                                                                                                                                                 |                                                                                                                                                        aGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAATCGAAACTGACACCGTATCTCGACGAACGGGGGTTTAACCTTGTGGGATACGGTTGTACCGCCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATTGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                                                                               <  1:81079/251‑1 (MQ=255)                                                      cAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACTGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatccn                                                                                           <  1:31079/250‑2 (MQ=255)                                                                                      cACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAACAGCGAAACTGACACAGTCTCTCGAAGAACTGGGATTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgccg                                                          >  1:235461/1‑251 (MQ=255)                                                                                                                                              ctcGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGATGCCCGATCCTATCGCAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGCAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGcc  >  1:218412/1‑251 (MQ=255)                                                                                                                                                                                   gTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGGAATCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGAAACCGTAACTTTGAAGGCAGTATCCATCCGCTGGCTAAAACTCCCTGGCTGGCCTcg                                                                >  1:98533/1‑152 (MQ=255)                                                                                                                                                                                                        ctGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggc                                                                        >  1:15693/1‑123 (MQ=255)                                                                                                                                                                                                            gCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGGCGGT‑‑GGTGCGGTCCGGCATCCTTAACTTTGAAGGCCGTATCCATCCGCGGTTTAAAACTAACTGGCTGGTCTcgccgcc                                                           <  1:63324/130‑1 (MQ=255)                                                                                                                                                                                                              cGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGTTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcg                                                                <  1:250807/125‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                        AGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCC  >  NC_000913/1337154‑1337545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑