Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	726,513	G→A	S175F (TCC→TTC)	kdpB ←	K(+) transporting P‑type ATPase subunit KdpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	726,513	0	G	A	100.0%	16.7 / NA	8	S175F (TCC→TTC)	kdpB	K(+) transporting P‑type ATPase subunit KdpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCAATAACTTCACCATCGCAGGGGATAATATCGCCAGCTTCTACCAG  >  NC_000913/726306‑726640                                                                                                                                                                                                                |                                                                                                                                tcgGAAAACGGCCACAGCGTGGCGTTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGTGCAGGGCAATTTCGTTCGGCGGTTTGCGTCGCTGTGCCCCTTCCCCCATCGCGATCACCCGATCCATAAAGGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGGCATTCATAAAGAATACGCGTGCCGCCGGTGACGAAGGCAAAATCGCCGCCGGATTCACTGATCACCGGTGc                                                                                            <  1:173158/244‑1 (MQ=255)  cgGAAAACGGCCACAGCGGGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGAAATCAGCAGAATGGTCAGGGCAATTTCTTTCAGCGTTTTGCGTCGCTGGGCGCCTTCCACCATCGCGTTCAACCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACGCAACCACCAGCCATTCAGAAAGAATACGCTTGCCGCCGGTGACGAAGTCAAAATCGCCGCCGGATTCACGGATCACCGGTCCCGATTcc                                                                                     <  1:101657/251‑1 (MQ=255)                             ccAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATAATCCGCTCCAGAAATGTCCCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGAAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGAt                                                          >  1:122766/1‑251 (MQ=255)                                                cAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGAAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCCATAAc                                         >  1:196852/1‑249 (MQ=255)                                                                                     ggCGTTTTGCGTCGCTGTGTGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGAAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCTATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCAATAACTTCACCATCGCAGGGGATAATATCGCCAGCTTCTACCAg  <  1:123684/251‑1 (MQ=255)                                                                                                          ccTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATAACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGAAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCAATAACTTCATCATCGCAGAGGATAATATCGCCAGCTTCTAcc    >  1:36386/1‑228 (MQ=255)                                                                                                                    tcgtgATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTNNCGAAGGNna                                                                                                                           >  2:7012/5‑99 (MQ=255)                                                                                                                                                                                              cgcgTGCCGCCGGTGACGAAGGCAAAATAGCCGCCGGATTAACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCAATAACTTCACCATCGCAGGGGATAATATCGCCAGCTTCTAcc    >  1:94351/1‑144 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                GCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAGGCAAAATCGCCGCCGGATTCACGGATCACCGGTGCCGATTCCCCGGTGATGGCGCTTTCATCGACCGATGCACCCCCTTCAATAACTTCACCATCGCAGGGGATAATATCGCCAGCTTCTACCAG  >  NC_000913/726306‑726640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑