Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	135,985	G→A	T160T (ACC→ACT)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	135,985	0	G	A	100.0%	26.5 / NA	11	T160T (ACC→ACT)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

ACCGCCGTAATAGGTCGGGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCAC  >  NC_000913/135778‑136146                                                                                                                                                                                                                |                                                                                                                                                                  aCCGCCGTAATAGGTCGGGATCGCCGCCTGTTAAAAGCCAACGTCGCTCAAGTAATGGCTGAGTTTGAGATATCTGTCGATGGCTTCTTCCTCCTGTACAAAGTAGACGCCGTTTTGGGCGACGCAGATACCGCCCGGAAACAGGCAACGTTTGCAGCCTTCATAAAATGCGGAAGCGAAAAGGCTTTCGACGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCCGGTTTGAt                                                                                                                        >  1:45013/1‑251 (MQ=255)                           ccTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGACAGGATTCAGGCAACGTTTGCAGCCTTTATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATc                                                                                              <  1:69789/251‑1 (MQ=255)                                                       aTGGCTGCGTTTGCGATGTCTGTCGAGTGCTTCTGCCTGCTGTAAAAAGCAGGCGCCGTTGTGTGCTACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCCAAGTTAAAAGGCGTTCTCCGGCGTCGAGAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGTGTGCTCTGACTTGCGAAATTGACGAGATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGc                                                                  <  1:43237/251‑1 (MQ=255)                                                              aGTTTGCGATGGCTGTCGATGGCTTCTGCCTGCTGTAAAAATCAGACGCCGTTTTGTGCGACGAAGATACCGCCATGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCTGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCggn                                                           <  1:65637/251‑2 (MQ=255)                                                                    cGATGGCTGTCGATGGATTCTTCATGCTGTAAAAAGCAGAAGCCGTTTTGTGAGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTa                                                                    >  1:209696/1‑236 (MQ=255)                                                                                                                gTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAACATGCCGAAGTGAAAAGGCTATCGCCGGGACCGATAGGATCAGTGCCATCGGCGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGc                                                                                                         >  1:216959/1‑155 (MQ=255)                                                                                                                                         ggATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGTTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGAtn                                                                          <  1:54742/161‑2 (MQ=255)                                                                                                                                                   aaCGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGTTTTGATTAACGAAATTGACGCCATCGTCGATCAACAGCTTAAAGCGCGGAtc                                                                          <  1:36875/151‑1 (MQ=255)                                                                                                                                                             gCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATCATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCAACAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGCCGGCAGAACGATACGACACCCGCATCGATTTccac  >  1:230826/1‑213 (MQ=255)                                                                                                                                                                          gCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCAc                                                                                            <  1:217144/110‑1 (MQ=255)                                                                                                                                                                                  gAAAAGGCTTTCGCCGGGACCGATAGGATCAGTGCAGTCGGAGATAATGACATAAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGc                                                                                        >  1:140977/1‑106 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                  ACCGCCGTAATAGGTCGGGATCGCCGCCTGATAAAAGCCAACGTCGCTGAAGTAATGGCTGAGTTTGCGATGGCTGTCGATGGCTTCTTCCTGCTGTAAAAAGCAGACGCCGTTTTGTGCGACGAAGATACCGCCAGGATTCAGGCAACGTTTGCAGCCTTCATAAAATGCCGAAGTGAAAAGGCTTTCGCCGGGACCGATAGGATCGGTGCAGTCGGAGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCAC  >  NC_000913/135778‑136146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑