Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,400	G→A	intergenic (+32/‑33)	yggX → / → mltC	putative Fe(2(+))‑trafficking protein/membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,400	0	G	A	100.0%	44.9 / NA	17	intergenic (+32/‑33)	yggX/mltC	putative Fe(2(+))‑trafficking protein/membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/5);  total (12/5)

GTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAA  >  NC_000913/3104155‑3104596                                                                                                                                                                                                                                                      |                                                                                                                                                                                                     gTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATa                                                                                                                                                                                                  >  1:3905/1‑250 (MQ=255)                     aaCGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCCCAAGAAAACCATGCTGATTAATGAAAAGAAACTAAACATGATGCCTGCCGAGCCCCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCAcc                                                                                                                                                                             >  1:123215/1‑251 (MQ=255)                                           cAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATAtctc                                                                                                                                                       >  1:100681/1‑251 (MQ=255)                                                   ccTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGc                                                                                                                                               >  1:134850/1‑251 (MQ=255)                                                                   gcaCAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTg                                                                                                                               >  1:15136/1‑251 (MQ=255)                                                                    cacaAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTTGCTTTGATTTCGCCGTTGc                                                                                                                              <  1:196413/251‑1 (MQ=255)                                                                                                                  aTGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGTCGATACCTATAACGAAGc                                                                                <  1:73041/251‑1 (MQ=255)                                                                                                                            cggCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGACGATAAACAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCt                                                                                                                             >  1:236082/3‑196 (MQ=255)                                                                                                                                   ntgctTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATAGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAaacacaa                                                                                                                                                                                     >  1:77241/2‑133 (MQ=255)                                                                                                                                     nctTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTg                                                                                                                    >  1:154782/2‑196 (MQ=255)                                                                                                                                                                      gggTAAAGAGGTGAATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGt                                                                          >  1:56727/1‑205 (MQ=255)                                                                                                                                                                       ngTAACGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAAc                                                                                     >  1:65729/2‑193 (MQ=255)                                                                                                                                                                                   cATATNNAGNNNTCNNCGCCGGAAGATAAAAAATAAAAANAGTGCCGGAGCACGCCTCCGGCAACTTACNTNANAACANNCACNACACGCACCCNGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCANCNCCTGTTCGACGACCAAAAAAGGCGATACCTANAACGAAGCCTggg                                                                           >  2:184459/1‑191 (MQ=255)                                                                                                                                                                                   cATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGTAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACTCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATGGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTggg                                                                           <  1:184459/191‑1 (MQ=255)                                                                                                                                                                                         gANNNNNATANGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTg                                                                                                                    <  2:154782/144‑1 (MQ=255)                                                                                                                                                                                                 taCGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTACATAACACCAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAACAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCaa  >  1:133122/1‑250 (MQ=255)                                                                                                                                                                                                              aaaaaataaaaaCAGTGCCGGAGCACGCCTCCGGCAACTTACATAAAAACAAACACAACACGCACCCGGAGGGGTGAAAAAATATCTCGCGCTGGCTGTGATTGCGCGGTTGCTCATCGCCTGCTCGACGGCCAAAAAAGGGGATACCTATAACTAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTga                    <  1:248599/219‑1 (MQ=255)                                                                                                                                                                                                                                                     tNCANAAAAANAAACNCAACACGCACCCGGANTGATGAAAAAATATCTCGCNNTNGCTTTNATTgc                                                                                                                                      <  2:100681/66‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                     GTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCTCCAAAGAAGCCTGGGCGCAGTGGCAGCACAAGCAAACCATGCTGATTAATGAAAAGAAACTCAACATGATGAATGCCGAGCACCGCAAGCTGCTTGAGCAGGAGATGGTCAACTTCCTGTTCGAGGGTAAAGAGGTGCATATCGAGGGCTATACGCCGGAAGATAAAAAATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAA  >  NC_000913/3104155‑3104596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑