Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,635	G→A	T3T (ACG→ACA)	yqhH →	lipoprotein YqhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,635	0	G	A	90.9%	24.8 / ‑5.9	11	T3T (ACG→ACA)	yqhH	lipoprotein YqhH
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (5/5);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATGAGCAAAAGGTCAATCAGCTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGC  >  NC_000913/3158390‑3158759                                                                                                                                                                                                                                                      |                                                                                                                             ngagaAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAAtttt                                                                                                                         >  1:53543/2‑251 (MQ=255)           gccgATTACGCCCGCATTCATGTTCGTTTTACGCTGACAAATGGCAACACCGAAGAGCAAGAACTAGTTTTACAGCGACGAGAAGGAAAGAGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGAAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCCGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTggg                                                                                                                >  1:230666/1‑250 (MQ=255)                ttCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGGGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATTTTCACCGTGGGAGCtgt                                                                                                          >  1:9846/1‑251 (MQ=255)                                              gACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTCCTTAAGCCGATTGAGGCAAAAACTGCCGCCAGGTTAAAGCAATGAGCTGACTTAAATAACAATTAGCCGGAACACTAAATAAAAGGGAACACTATATGAAAACAATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTg                                                                            >  1:132990/1‑251 (MQ=255)                                                      gCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGAGTAAAGCAATGATCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATGTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATg                                                                    <  1:136049/251‑1 (MQ=255)                                                                            ggTTTTACAGCGGCGCGAAGGCAAGTGGGACATCGCTGATTTTATCCGCCCGAACAGCGGCAGACTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAACAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATGAGCAAAAGGTCAATCAGCTGGc                                              >  1:222332/1‑251 (MQ=255)                                                                                                gCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAAGTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATTTTCAcc                                                                                                                     <  1:217085/160‑1 (MQ=255)                                                                                                   aGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATTTTCACCGTGGTAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATGAGCAAAAGGTCAATCAGCTGGCGAGCAATGTGCAAACATTAAATg                       <  1:131646/251‑1 (MQ=255)                                                                                                                          ctcccGAACAGCGGCAGCCTACTTAAGCAGATTACGGCCAAAACTGCCGACCGATTAACGCAGTGCGATGGATTAAATAACAATTAGCCGGAACAATAATTAATAGGGAACACTATATGAAAACACTTTTCACCGTGGGAGGTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCCATGAGCAAAAGGGCAATCAGCTGGCGAGCACTGTGCAAACATTAAATGCCAAAATCGACCGGCTTGAga  >  1:222152/3‑248 (MQ=255)                                                                                                                                                            aGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCGGAATTAAATAACAAGTAGCCGGAACAAGAAAGAAAAGTGTACACTGTATGAAAACAATGTTCACCGTTGGAGCGGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATGAGCAAAAgg                                                           <  1:170341/158‑1 (MQ=255)                                                                                                                                                                                   aaGCAANNAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACAATTTTCACCGTGGGAGCTGTTGTTCTgg                                                                                                  <  2:9846/96‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                             GGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTTTCACCGTGGGAGCTGTTGTTCTGGCAACCTGCTTGCTCAGTGGCTGCGTCAATGAGCAAAAGGTCAATCAGCTGGCGAGCAATGTGCAAACATTAAATGCCAAAATCGCCCGGCTTGAGC  >  NC_000913/3158390‑3158759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑