Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 767,857 G→A G625D (GGC→GAC)  mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913767,8570GA90.9% 20.1 / ‑6.2 12G625D (GGC→GAC) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (4/6);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAG  >  NC_000913/767625‑768052
                                                                                                                                                                                                                                        |                                                                                                                                                                                                   
gggAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTCGGTGCTATGAATATCGGACTTTCGACACCCGGTGCCGGCATTTTCTCTCTCTTTTTCCTTCATGATAATGGCGCGGGCGGTGTTCTGGCTGGAATTGGCTGGTTTTTCGCGGCCTTTGTgggggg                                                                                                                                                                                   >  1:45775/1‑250 (MQ=255)
                                             gCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTTGGCTCTATGGTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCTGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTTGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGt                                                                                                                                      <  1:192132/251‑1 (MQ=255)
                                                                  gattcGCTGCGGGTTATTGGTTCGTTTGTGCTGTGCTCTATGGTAACGGGCGCTAGTGGCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACATCATTATAATGGCGCGGGCGGTTGGAGGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAAt                                                                                                                                                                 <  1:136092/199‑1 (MQ=255)
                                                                                                           ggTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTAATGATAATGGCGCGGGAGGGGTTATGGCGGCAATTGGCTGGTTTGACGCGGACTTGGGGGGGGCTGCAATCTCGACTGCAATTCTTCTGATGTGGCGGGGTCACGCGGTTTAGCATGGCAACTCTCTGACTGATGGCGTCATTCCCTAAACAAAAAGGAAACGACGATGa                                                                         >  1:163434/1‑250 (MQ=255)
                                                                                                                                  cGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGCTAATGGCGCGGGCGGTGTTCTGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCCATCTCGACTGCAATTCTCCTGACGTGGCGGCGCCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGc                                                                                                   >  1:141998/1‑201 (MQ=255)
                                                                                                                                                               gCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGACGCGGGATTGGTGGGGGCTGCAATCTCGGCTGCAATTCTCCTGGTGTGGCGGCGTCCCGCGGTTAAACATGGGAACTATCTGACTGATGGGGGAATGCCAAAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCCCGTTCACATCACCCCCCAAATGCACTGGGATCGGGAGTgg                    >  1:125001/1‑251 (MQ=255)
                                                                                                                                                               gCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTTGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTTATGTCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTgg                    <  1:220915/251‑1 (MQ=255)
                                                                                                                                                                            cGCTCTTTTTTCTTCAGGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGTAATCTCGACCGCAATTCTCCTGATGTGGCGGCTTCACGCGGTTAAGCATGGCAACTAGCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGGATCGCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTcaccac         <  1:238046/249‑1 (MQ=255)
                                                                                                                                                                                  ttttACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAagan  <  1:150150/250‑2 (MQ=255)
                                                                                                                                                                                                 aTGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTTGTGGGGGCTGCAAGCTCGACTGCAATTCTCCTGACGTGGCGGCGTCACGCGGTTAAGCATGGCAACTAGCTGACAGAGGGCGTAAGGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATc                                                  <  1:39236/187‑1 (MQ=255)
                                                                                                                                                                                                          ncggTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGGAACTATCTGACTGATGGCGGAATACCCTAAAc                                                                                            >  1:154625/2‑136 (MQ=255)
                                                                                                                                                                                                          gcngTGTTATGGCGGCAATTGGCTGGTTTGACGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAAc                                                                                            <  2:154625/136‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                   
GGGAAATCCACCTGGCTGTTAGGGCTGGCAGGTATTACCGAAGGGGCGATCCCGATGGCGATTGAAGATCCGCTGCGGGTTATTGGTTCGTTTGTGCTGGGCTCTATGGTAACGGGCGCTATTGTCGGTGCGATGAATATCGGCCTTTCGACACCCGGTGCCGGCATTTTCTCGCTCTTTTTACTTCATGATAATGGCGCGGGCGGTGTTATGGCGGCAATTGGCTGGTTTGGCGCGGCATTGGTGGGGGCTGCAATCTCGACTGCAATTCTCCTGATGTGGCGGCGTCACGCGGTTAAGCATGGCAACTATCTGACTGATGGCGTAATGCCATAAACAAAAAGGAAACGACGATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAG  >  NC_000913/767625‑768052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑