Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	823,314	C→T	E142E (GAG→GAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	823,314	0	C	T	90.9%	20.8 / ‑5.1	11	E142E (GAG→GAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (3/7);  minor base A (0/1);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGCTTTAACC  >  NC_000913/823116‑823554                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 aaTATCATAGCGATGATCTTCGTTATCGCGCCAGACAAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTACGCTTTGGGAAGACGTAGCCACCAGCGGCGAGCCGCGCTATGTCCAGGCAGGGTTTCCAGCTCAAACCGGAGAATATCTTCGACCATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTTGCTTTTGGTCCGTCGCTTGAACTATGCTCGGCGGAGTAATTAAGCCCGCCaaaa                                                                                                                                                                                               >  1:10304/1‑248 (MQ=255)                                      aGCAATCCCGGCGCTTCTCCCGCCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATACCTGCGACAATCGGCCCTTCAAGGCTTGCCGCGTAATCCTGTTTGGCATCTGGACCGTATCTAGACATATACTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATAcgcg                                                                                                                                                                                  <  1:244722/226‑1 (MQ=255)                                                                                                             cgcgcttggtccatgtgggTTTTCCAGCTCAAACTGGGAAATTTGTTCGACAATCGGCCCTTAAATGCGTCCCGCGAAATCTTTGTTAGCTTCTGGACCGTAGCTGGACATATGCGCGGCGGAGTAATTCATCCCGCCAATAAAGGCTAGACGCGCGTCGATCACCACAATTGTGCGATGCTTCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGc                                                                                  <  1:221838/234‑1 (MQ=255)                                                                                                                          ggcaggTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCTTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGc                                                                                                                                                                                                        >  1:75154/1‑120 (MQ=255)                                                                                                                                 tttCCAGCTAAAACGGTAGAATATCTTCGACAATCGCCCCGGCAAGGCGTACCGCTTAATCCTGTGTAGCTTCTGGACCGTAGCGGGACATATGCTCGGCGTAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTGGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCAtt                                                               <  1:118086/250‑1 (MQ=255)                                                                                                                                       gCTCAAACTGGAAAATATCTTCAACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCTTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGc                                                                                                                   <  1:202286/192‑1 (MQ=255)                                                                                                                                            aaCTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCTTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAgn                                                                              <  1:68063/224‑2 (MQ=255)                                                                                                                                              cgggAGAATATCTGCGACAATCGGCCCTTAAAGGCGTGCCGCGTAAGCCTGTTTAGCTTCTGGACCGTAGCTGGACATATGCTGGGTGTAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGc                                                 <  1:124697/249‑1 (MQ=255)                                                                                                                                                                   cGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCTTCTGGACCGTAGCTGGAAATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAgn                            <  1:76215/251‑2 (MQ=255)                                                                                                                                                                    ngCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCTTCTGGTCCGTAGCTGGAAATATGCTCGGCGGAGTAATTCAGc                                                                                                                                                                                                        >  1:32333/2‑78 (MQ=255)                                                                                                                                                                                             ccTGTTTATCTTTTGGCCCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGCTTTAAcn  <  1:204159/251‑2 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 AATATCATCGCGATGTTCTTCGTTATCGCGCCAGACCAGCAATACCTGCGCTTCTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGGCGAACCGTAGCCATCCAGCAAGACTTCCGCTTTAACC  >  NC_000913/823116‑823554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑