Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,945	G→A	T93I (ACC→ATC)	tyrS ←	tyrosine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,945	0	G	A	91.7%	24.1 / NA	12	T93I (ACC→ATC)	tyrS	tyrosine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (7/4);  minor base C (1/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GAACGAAATCCCCTGATCTTCACGGTTGAGACGCTGCTTAACCGCTTCTTTGTTGATCATCTGGTTAACGGAGAAGTGTTTGCCAATATCGCGCAGGAAGGTCAGCACATTCATATTGCCGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGC  >  NC_000913/1716713‑1717128                                                                                                                                                                                                                                         |                                                                                                                                                                                        naaCGAAATCCCCTGATCTTCACGGTTGAGACGCTGCTTAACCGCTTCTTTGTTGATCATCTGGTTAACGGAGAAGTGTTTGCCAATATCGCGCAGGAAGGTCAGCACATTCATATTGCCGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCgg                                                                                                                                                                       >  1:156921/2‑251 (MQ=255)                                                                                            cgcAGGAAGGTCAGCACATTCATATTGCCGAACCAGTCATAGTTGGTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCACGGCAGCTTTGAAGCTCGTGTCGCCAATCAGTCCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCa                                                                            <  1:220837/251‑1 (MQ=255)                                                                                                ggAAGGTCAGCACATTCATATTGCCGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCCCTCCTGAACAGTTTCTTCGATGTTCAGc                                                                                                                                                                                 >  1:131680/1‑146 (MQ=255)                                                                                                          cacaTTCATATTGCCGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTa                                                                                                                                                                              >  1:82208/1‑139 (MQ=255)                                                                                                                       ncGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCg                                                                                                                                                                        >  1:53222/2‑132 (MQ=255)                                                                                                                                               gcgATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGc                                                                                                 >  1:14437/1‑179 (MQ=255)                                                                                                                                                                   nnnnnnnnnnacNNNNNACGNGGCAACCTGCTTTCGGATTTTGGCCCCCCCCTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGGCGCCAAACAGGCCCGTCGCGCCGCCTACCAACGCAACCGGCTTGTgg                                                                                                      <  2:156921/143‑1 (MQ=255)                                                                                                                                                                     aGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCGGCAGCTTTAAAGCTCGGGTCGCCAAGCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGGTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAgcg           <  1:249362/243‑1 (MQ=255)                                                                                                                                                                           aaTCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTCAACAGCTTCTTCGCTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGACCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGACCAAGATGCCCCAAATGCAAGCTGTCAGCGGGAGGATCGAAGCCGCAATAGAGCGCGATCGGGc  >  1:130818/1‑246 (MQ=255)                                                                                                                                                                                                             gTCCANCCACCNCNNGACAGTTGCTTCGATGTTCNGCTTACGCTCGGCAGNTTTGNAGCTCngtnc                                                                                                                                                    >  2:98166/1‑63 (MQ=255)                                                                                                                                                                                                               ccacccacTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCgcgc                                                                                                                                >  1:35026/1‑84 (MQ=255)                                                                                                                                                                                                                cacccacTCCTGAACAGTTTCTTCGATGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGcc                                                                                                    <  1:224217/111‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                        GAACGAAATCCCCTGATCTTCACGGTTGAGACGCTGCTTAACCGCTTCTTTGTTGATCATCTGGTTAACGGAGAAGTGTTTGCCAATATCGCGCAGGAAGGTCAGCACATTCATATTGCCGAACCAGTCATAGTTGTTCGCCGCGATAGCAGAGTTTTCTCCACAGTCGAAATCGAGGAACGGGGCAACCTGCTTACGGATTTTGTCCACCCACTCCTGAACAGTTTCTTCGGTGTTCAGCTTACGCTCGGCAGCTTTGAAGCTCGGGTCGCCAATCAGACCCGTCGCGCCGCCTACCAGCGCAACCGGCTTGTGGCCCGCCTGCTGGAAGCGTTTCAGGCATAACAATGGAACAAGATGCCCCAAATGCAAGCTGTCAGCGGTAGGATCGAAGCCGCAATAGAGCGCGATCGGGC  >  NC_000913/1716713‑1717128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑