Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	794,092	G→A	P185S (CCG→TCG)	modE ←	DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	794,092	0	G	A	100.0%	28.6 / NA	12	P185S (CCG→TCG)	modE	DNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

GCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTG  >  NC_000913/793859‑794275                                                                                                                                                                                                                                          |                                                                                                                                                                                        gCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGACGAGAAGTCGCTTCATTTACCGGCCCTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATc                                                                                                                                                                         >  1:136926/1‑250 (MQ=255)                                                         cgtGCTGAAGAGATGTCGCTTCATTTACCGGCACGTTGGCGCACAGTCTTGGCCCGTCGGGTAGCTCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATTCCCGGGAATGGGTTGTCAGCGTGTTGCACGACCGCCTCGTCCTGAGTAATACCTACCCACGACTCTTTTAGCAATATCAACACTTTTCTGCCTTCATCCAGCCCCAGCCGCGCGCCGCTTTGTGCGGt                                                                                                                       <  1:177538/242‑1 (MQ=255)                                                                                      ggCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAg                                                                                       >  1:164779/1‑247 (MQ=255)                                                                                                                        ncgcCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCACCATGCTGTTGAACGTCATCATg                                                  >  1:66413/2‑250 (MQ=255)                                                                                                                                    cTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTGGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCAGCCAGCCCCAGACGCGCGCCGCTGTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGtcatca                                                    <  1:98866/236‑1 (MQ=255)                                                                                                                                         cACTGCTCTGCGCCGCGCTCAATATGACTAATAATCCCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATCAACACTTCtttn                                                                                                                                                             <  1:68796/126‑2 (MQ=255)                                                                                                                                                                    cTAATAATACCCGTTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTGCTGAGTAATACCTACCCACGACGCTTATAGCAATAGCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCgg                                                                                                                        <  1:221861/136‑1 (MQ=255)                                                                                                                                                                       aaaataCCCGGTAATTGGTTGTCAGCTCTTTGCGCGACCGCCTCGGCATGAGTAATTCCTACCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGACGCGTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCTTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTg  <  1:193140/249‑1 (MQ=255)                                                                                                                                                                                        gTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGAcgc                                                                                                                                        <  1:195911/100‑1 (MQ=255)                                                                                                                                                                                           gTCAGCGTTTTGCGCGACCGCCTCGTCCTCAGTAATACCTACCCACGACGCTTTTAGCAATATCCACACTTCTTTGCCTTCATCCAGCCCCAGAcgcgcg                                                                                                                                     >  1:84156/1‑100 (MQ=255)                                                                                                                                                                                                                      cggAGTAATACCTGCCCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGAcgcg                                                                                                                                       <  1:220620/69‑1 (MQ=255)                                                                                                                                                                                                                                cctaccCACGACGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGCAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATcc                                                                         >  1:13563/1‑122 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                        GCACAGCGTGGCGATAATCACGCTGTCGGCATTAAAGTAGGCCGTGACATTCTGTCCTTGCTGAAGAGAAGTCGCTTCATTTACCGGCACTGTGGCGCACAGTGTTTGCCCGTCGGGTAGCGCCATTAATACTTCGCACTGCTCTGCGCCGCGCTCAATATGACTAATAATACCCGGTAATTGGTTGTCAGCGTTTTGCGCGACCGCCTCGTCCTGAGTAATACCTACCCACGGCGCTTTTAGCAATATCAACACTTCTTTGCCTTCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTG  >  NC_000913/793859‑794275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑