Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,807,994	C→T	A288V (GCC→GTC)	proX →	glycine betaine ABC transporter periplasmic binding protein ProX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,807,994	0	C	T	100.0%	24.8 / NA	10	A288V (GCC→GTC)	proX	glycine betaine ABC transporter periplasmic binding protein ProX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

GTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGCATCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATC  >  NC_000913/2807775‑2808224                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       gTAACGAACTGAAGCCGGGCACAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCACACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGcc                                                                                                                                                                                                         >  1:208609/1‑251 (MQ=255)           gAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTGCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGGCAACAAAGCCGGGGCCGAGAAAAGCCCGGCAGCAGCGGAGCTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGc                                                                                                                                                                                                <  1:225244/250‑1 (MQ=255)                                ggggTTGCAGGTGCCGGTCTCCCCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAAAAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTATTTGCCATTATGCAGTGGACAGTGGCAGATATTACCGTCCAGAACGCCATTATGCATGACGGCAAAGCCTAAGAAGGCGATATTCAGGGACACGTTGATg                                                                                                                                                                          <  1:92206/249‑1 (MQ=255)                                                                            ncGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAAt                                                                                                                              >  1:65817/2‑251 (MQ=255)                                                                                                                                            caacaaAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAAAGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGGGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGCATccgcc                                                              >  1:175848/1‑251 (MQ=255)                                                                                                                                              acaaAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAACGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGCATCCGCCGt                                                            >  1:105392/1‑251 (MQ=255)                                                                                                                                                                   ncccGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCAAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCg                                                                      >  1:160250/2‑220 (MQ=255)                                                                                                                                                                   aCCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCATGGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAg                                                                                                                           <  1:144732/167‑1 (MQ=255)                                                                                                                                                                                           ccATTATGCAGTTGCCAGTGGCAGATATTAACGTCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGc                                                                     >  1:169200/1‑197 (MQ=255)                                                                                                                                                                                                        gCCAGTGGCCGATATTAACGTCCAGACCGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCCACAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGCAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGCATCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAAACACATc  >  1:228454/1‑251 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       GTAACGAACTGAAGCCGGGCAAAGATGTCGTCTGGTTGCAGGTGCCGTTCTCCGCACTGCCGGGCGATAAAAACGCCGATACCAAACTGCCGAATGGTGCGAATTATGGCTTCCCGGTCAGCACCATGCATATCGTTGCCAACAAAGCCTGGGCCGAGAAAAACCCGGCAGCAGCGAAACTGTTTGCCATTATGCAGTTGCCAGTGGCAGATATTAACGCCCAGAACGCCATTATGCATGACGGCAAAGCCTCAGAAGGCGATATTCAGGGACACGTTGATGGTTGGATCAAAGCCCACCAGCAGCAGTTCGATGGCTGGGTGAATGAGGCGCTGGCAGCGCAGAAGTAATTTTTATTCGGGCGGATAAGGCGTTTTTGCCGCATCCGCCGTTCTGTGCACAATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAACCACATC  >  NC_000913/2807775‑2808224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑