Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,040,041	G→A	E63K (GAA→AAA)	fldB →	flavodoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,040,041	0	G	A	100.0%	28.4 / NA	12	E63K (GAA→AAA)	fldB	flavodoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

CGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACGGAAGGATATGAATTTACCAG  >  NC_000913/3039820‑3040237                                                                                                                                                                                                                              |                                                                                                                                                                                                     cGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCGCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGTGAAGTCGTCTGGGATCAGCTc                                                                                                                                                                         <  1:84059/251‑1 (MQ=255) cGCAAAAGCCACAGAAGAATCGAGAAGATTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATCAGCTc                                                                                                                                                                         >  1:210630/1‑251 (MQ=255)                           gCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGCAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAAATGGTGACCTTACATAACCTCAAGGACGCCTCCCCGCAATTAATGGACCAGTCCGAAGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAt                                                                                                                                               >  1:138837/1‑251 (MQ=255)                               aCGTTATGAATATGGGTCTTTTTGACGGTTCCAGCACCTGTTACACCGAAATGGCGTCAGAAAAAATCCGCGATATTATCGGCTCAGAACTTGTGACCTTACATAACCTCAAGGACGACGCCCCGAAATTAATGGAGCAGTACTATGTGCTCATTCTGGGTATCCCGACCTGGGAGTTTGTTGAAATCCAGAAACACTTGGAAGCCGTCTGCAATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttgt                                                                                                                                            <  1:110383/250‑1 (MQ=255)                                          atGGGTCTTTTTTACGGTTCCAGCTCCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATg                                                                                                                                 <  1:190153/250‑1 (MQ=255)                                                                                                  ccGCGATATTATCGGCCCATAACTGGTGACCTTACATAACCTCAAGGACGACGCCCCGAAATTAATGGAGCAGTACGATGTGCGCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATCAGc                                                                                                                                                                           <  1:214734/152‑1 (MQ=255)                                                                                                               ggCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTT‑GGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATc                                                                                                                                                                              >  1:227003/1‑135 (MQ=255)                                                                                                                             gACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAATGTAAAATTGTTGCGCTGTATGGGCTTGGCTAGCAGTTGGGATACGTCGAGGAGGTCCTCGATGCGCTCGTTATGCTGCATGACAAACTCTCGACCAAAGGCGTGa                                             <  1:104835/251‑1 (MQ=255)                                                                                                                                  tACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGAAAGACTGTGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGtt                                         <  1:7668/250‑1 (MQ=255)                                                                                                                                               ggACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGCCCTGGGATTTTGGTGAAATCCAGAAAGACTGGGAAGCCGTCTGGGATAAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTAGACCAAAGGAGTGAAGTTCGTCGGc                                  >  1:34710/1‑244 (MQ=255)                                                                                                                                                                       ncAGTACGATGTGCTCATTCTGGGTATCACGACCTGGGATTTTGGTGAAATCCCGACAGACTGGGAACCCGTCTGGCATACCCTCGAAAACCTCAACCTTGAAGGTACACTTGTTGGGCTGTATGGGCTTGACGACCAACTGGGATACGGCGAGTGGTTCCTCGATGCGGTCAGTATGCTGCATGACAAACTCTCGACCAAAGAAGTACAGTTCGTCGGCTACTGGCCAACGGAAGGATATGAATTTAcc    >  1:27300/2‑250 (MQ=255)                                                                                                                                                                        cAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAAGCCAGAAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGTCGAGTGGTTCCTCGATGCGCTCTGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCTTCGGCTACTGGCCAACGGAAGTATATGAAGTTACCAg  <  1:98536/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                     CGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACGGAAGGATATGAATTTACCAG  >  NC_000913/3039820‑3040237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑