Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,133	C→T	W431* (TGG→TGA)	hypF ←	carbamoyl‑‑[HypE] ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,133	0	C	T	93.8%	34.0 / NA	16	W431* (TGG→TGA)	hypF	carbamoyl‑‑[HypE] ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (10/5);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑CCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTG  >  NC_000913/2835897‑2836357                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 ccGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGAGGCCACTGATGATCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGGGTCATGCCCAACGTATTGCGGGGGAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCGTCCA‑TGCATCTGGAt                                                                                                                                                                                                                    >  1:172886/1‑251 (MQ=255)            cATACCGAGACCGTCGAGCGCCAAAGCAATGTCATCACCGCCAGCCAGCTGCCACTGATGCTCTGCCAGTCACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGAGGTTGTGCATCAGGCGTAACGCTGCGCG‑TCACTGCATCTGGATGCCATCGTCAc                                                                                                                                                                                                         <  1:104552/251‑1 (MQ=255)                  gataccgTCGAGCGTCAAAGCAATGACATCACCGCCACCCAGCGGCCACTGATGCTATGCCAGACACGCCGCTGCATGGGAATGATGCTGCAGTACCGTTTGCGTCGGCAGCTTCATTTCACGCGCCCACTAGCTGGAGCCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGCGTAAAATCGACGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACtt                                                                                                                                                                                                       >  1:161391/1‑247 (MQ=255)                        gTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGccc                                                                                                                                                                                              >  1:143426/1‑250 (MQ=255)                                        aTGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCTTGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTTAacac                                                                                                                                                                             <  1:201101/251‑1 (MQ=255)                                                aCCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGtt                                                                                                                                                                     >  1:214103/1‑251 (MQ=255)                                                     cATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCCTGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACcg                                                                                                                                                                >  1:175332/1‑251 (MQ=255)                                                                             ccAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCAAGCGCCCACTGGCTGGAGACATAGACCGGATGCGCGTCATGCACAACGTATTGCGGGGTACAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGCTGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTc                                                                                                                                        >  1:179985/1‑251 (MQ=255)                                                                               aGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGGCGGCAGAGTCATTTCACGCGCCCACGGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTGCGCG‑TCACGGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCGGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAg                                                                                                                                      <  1:111809/251‑1 (MQ=255)                                                                                        gcTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTCGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGcc                                                                                                                             >  1:221827/1‑251 (MQ=255)                                                                                                                             gCAGATTCATTCCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAAc                                                                                                        >  1:90586/1‑235 (MQ=255)                                                                                                                                             cgcCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACAttt                                                                                                    >  1:43564/1‑223 (MQ=255)                                                                                                                                                           agaCATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATTTTTTGCATCAGGCGTACCGCTTCGCG‑TCTCTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACTGCGTGTTCACGGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGac                                                                                                                        <  1:236220/189‑1 (MQ=255)                                                                                                                                                                                           tATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTCACGCTTCGCG‑TCATTGCATCTGGATACCATCGTCACTTAAATCTCCCAGCTGATGACTCAACACCCCTTGTTCACCGCGCACCCGGCAGAAGGCATTTTTCAGCACCGTGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAACACAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGAATTTCGCCGCTTTcgcg                          >  1:245646/1‑251 (MQ=255)                                                                                                                                                                                                  nggTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgc                           >  1:67066/2‑243 (MQ=255)                                                                                                                                                                                                                    tttGCATCAGGCGTAACGCTTCGCG‑TCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTg  <  1:98659/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 CCGTTCTCCCCCATACCGATACCGTCGAGCGTCAAAGCAATGACATCACCGCCATCCAGCGGCCACTGATGCTCTGCCAGACACGCCGCTGCATGGGCATGATGATGCAGTACCGTTTGCGTCGGCAGATTCATTTCACGCGCCCACTGGCTGGAGACATAGCCCGGATGCGCGTCATGCACAACGTATTGCGGGGTAAAATCGTAGATGTTTTGCATCAGGCGTAACGCTTCGCG‑CCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTG  >  NC_000913/2835897‑2836357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑