Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,517,508	G→T	V40V (GTG→GTT)	ortT →	orphan toxin OrtT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,508	0	G	T	100.0%	20.8 / NA	9	V40V (GTG→GTT)	ortT	orphan toxin OrtT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/6);  total (3/6)

GTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAG  >  NC_000913/1517360‑1517696                                                                                                                                                     |                                                                                                                                                                                             gTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCATGTTTTTTATGCGGTTATGGCCGCAATCGCATTTCTTATCACCTGGTTTCTTTCTTACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTTACGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCAATGGCTTGACGGcac                                                                                                               >  1:105422/1‑228 (MQ=255)                  ggACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTGCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCATGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTc                                                                        <  1:235937/250‑1 (MQ=255)                                               caTGCTTGTTTTTTATGCGGTTATGGCCGCACTCGCATTTCTTATCACCTGGTTTCTTTATCACGATAAGAAACGCATCAGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGCTGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATTTTACTCACTGGCTTGACGGCACCACGCCGACTTATCTCATTTGGATGAATCGATAAATTTCACAAGTGGc                                                               >  1:171191/1‑230 (MQ=255)                                                               gCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATcg                          <  1:180435/251‑1 (MQ=255)                                                                                         tATCTCCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTGCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATGTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAag  <  1:227760/249‑1 (MQ=255)                                                                                                             aCGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAAGTTCACAAGTGGCTAAGGAGAAAGTATGTc                                              <  1:74243/185‑1 (MQ=255)                                                                                                                                         cgcCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCa                                                                                                                                                 <  1:10638/58‑1 (MQ=255)                                                                                                                                         cgcCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGCGGCGCTGTTGTTTTCa                                                                                                                                                 >  2:10638/1‑58 (MQ=255)                                                                                                                                           ccTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCGCGCAGGCTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTg     <  1:239966/196‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                                                             GTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAG  >  NC_000913/1517360‑1517696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑