Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	869,891	C→T	P161S (CCG→TCG)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	869,891	0	C	T	100.0%	16.9 / NA	8	P161S (CCG→TCG)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAACTGGATACCTGGAATCAGACCGATTTTGTGAAGGTGAAAAAATTCGCGGGTTACTGGCAGCCAGGATTGCCCAAACTGGACAGCATAACCTGGCGTCCGGTGGCGGATAACAACACCCGCGCGGCAATGCTG  >  NC_000913/869664‑870109                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           gctttacttacACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATATCCTGTATAAGAATATTGCTAAAACTGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATTCGGCGACCGCGATGATTTCAccn                                                                                                                                                                                                     <  1:46879/248‑2 (MQ=255) gCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGAACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATTCGGCGACCGCGATGATTTCACCg                                                                                                                                                                                                     >  1:139631/1‑251 (MQ=255)                     gggCGGGAAAGCATTAAATTCCAGGCTGACACCGATTTCAACGCCGCGGCGGTTAAAGCGAATCTGGACAGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAACCGGAAGCGATCGATCCGACAACGGTAAAGTTTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCTTTCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATgg                                                                                                                                                                                 <  1:57302/249‑1 (MQ=255)                                                 gCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGTTCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTCTTAATATTCTTGCCCATTCGGCGACCGAGATGATTTCACCGGCAGCGCTGGAAAAATATGGCCAGGAGATTGGTTTTTATCCGGTggg                                                                                                                                                      >  1:126348/1‑250 (MQ=255)                                                                                                   gATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATTCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAACTGGATACCTGGAATCAGACCGATTTTGTGAAGGt                                                                                                   >  1:83014/1‑251 (MQ=255)                                                                                                                                                             ctatcgatcCGACAACGGTAAAGATTCCCCTCAAACAGGCGATCTCAGCGATTCTTAAAATTCTAGCCACTTCGGCGCCCGCGATCATTTCACCGGCAGCGCTGGCCAAATATGGCAAGGAGATTGGTTTTTCTCCGGTGGGAACCGGACCGTATGCACTGGATACATGGAATCCGACCGATTTTGTGAAGGTGAAAAAATTCGCGGGTTACTGGCAGCCAGGATTGCCCAAACTGGACAGCATCACCTgg                                         >  1:239419/3‑251 (MQ=255)                                                                                                                                                                                          cTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATTCGGCGACCGCGATGATTTCACAGGCAGCGCTGGCAAAATATGGCCAGGCGATTGGTTTTTATCCGGAGGGAACCGGACCGTATGAACTGGATACCTGGAATCAGACCGATTTtgt                                                                                                         >  1:144240/1‑158 (MQ=255)                                                                                                                                                                                                    cGTTCTCAGTGTTTATTAATATTCTTGCCCATTCGGCGACCGCGATGAGTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAGCCGGACCGTATGAACTGGATACCTGGAATCAGACCGATTTTGTGAAGGTGAAAAAATTCGCGGGTTACGGGCAGCCAGGAGTGGCCAAACTGGACATCATAACCTGGCGTCCGGTGGCGGATAACAACACCCGCGCGGCAAtgctg  <  1:215852/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                           GCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTTTATTAATATTCTTGCCCATCCGGCGACCGCGATGATTTCACCGGCAGCGCTGGAAAAATATGGCAAGGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAACTGGATACCTGGAATCAGACCGATTTTGTGAAGGTGAAAAAATTCGCGGGTTACTGGCAGCCAGGATTGCCCAAACTGGACAGCATAACCTGGCGTCCGGTGGCGGATAACAACACCCGCGCGGCAATGCTG  >  NC_000913/869664‑870109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑