Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,375	G→A	L21L (CTG→CTA)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,375	0	G	A	91.7%	20.6 / NA	12	L21L (CTG→CTA)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/5);  minor base C (1/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAAATTAATTCACAACAATTCAATGGCTTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGG  >  NC_000913/3041163‑3041573                                                                                                                                                                                                                     |                                                                                                                                                                                                       gcgaAATTAATTCACAACAATTCAATGGCTTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCCTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTTTACCGCTGACGCTAATGACGCTTGAAGACTGGGCGCTTGCCACCATTACTgg                                                                                                                                                                  >  1:193494/1‑251 (MQ=255)                           ggCTTCATTTTTTTGGAAGTCGCCTCGCATAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCTTTAAGCAACTCAGGACTCTATTATGGCTTTTTCACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTATACCGCTGACGCTAAGGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgt                                                                                                                                        <  1:215581/251‑2 (MQ=255)                           acggtgtgctcttccgatctCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgn                                                                                                                                        <  1:160347/232‑2 (MQ=255)                                cATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAgn                                                                                                                                                        <  1:28429/230‑2 (MQ=255)                                                                             aaaGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAAGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTAAGCATACGGCTTCTGCCCGTTTACCGCTGACGCTCATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCCGCACCTGCTc                                                                                      >  1:169166/1‑251 (MQ=255)                                                                                       cATTGCCTCCTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt                                                                            >  1:206005/1‑251 (MQ=255)                                                                                                                         aTACGCCGTTAAGCAACTCAGGACTCTATTATGGCGTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACg                                          <  1:36315/251‑1 (MQ=255)                                                                                                                                     gCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAACGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGa                                                                                                                                                      >  1:108867/1‑131 (MQ=255)                                                                                                                                                         ggCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGgcgc                                                                                                                                                                                  >  1:61385/1‑83 (MQ=255)                                                                                                                                                                 cacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTCGCCACCATTACTGGCGCGGACCGCGAAAAATCTATGCAGGGTCCGGTGCCAGCAGCTGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCCTTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACgg  >  1:127293/1‑251 (MQ=255)                                                                                                                                                                          ctccCCGTCCGGCTACAGCTTCTGCCCGTTTACCGCTGACGCTAATGGCGCTTGATGACTGGGCGCTTGCCAACCTTATTGGCGCGGACAGCGCCCAATATATGCAGGGTTCGGTGCCAGCAGGTGTACGCCAGATGGCa                                                                                                        >  1:39429/1‑140 (MQ=255)                                                                                                                                                                          cgccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTAATGACGCTTGATGACTGGGCGCTTGCCACCATTGCGGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccgc                                                                                 <  1:165258/161‑1 (MQ=255)                                                                                                                                                                                                       ntANCGCTGNCGCTNACGACGCTTGATGACNGGGCGCTTGCCACCATTACNNGNTCGGANAGCGa                                                                                                                                                      <  2:108867/64‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                       GCGAAATTAATTCACAACAATTCAATGGCTTCATTTTTTTGGAAGTCGCCTCGCAGAAGGCACAGATCGCGTAGTGAAAGCACTTGCATTGCCTCATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGG  >  NC_000913/3041163‑3041573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑