Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,529,464	G→A	I260I (ATC→ATT)	sgcC ←	putative PTS enzyme IIC component SgcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,529,464	0	G	A	90.0%	19.7 / NA	10	I260I (ATC→ATT)	sgcC	putative PTS enzyme IIC component SgcC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/5);  minor base C (1/0);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCATGAAAGGGATTTTTTCATAGATAGCATCAAGTAGAACGAAAAGCGGTACTGAACTG  >  NC_000913/4529234‑4529701                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              naGAACGGTGCTACCGGCAGGTCTGCGGGGGGTAACAATTTGTTGCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGGATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATAAACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAacac                                                                                                                                                                                                                           >  1:31147/2‑251 (MQ=255)          gCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAAAATTCTGGACCGAATTACCATAATAGGTAACAGTAGCAGACCGACAGCAATGGTGGTTGGACGCCCCAGCGTCACCGCCGGATCCAGGCAGATTTACACTTAACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCCACAATCAGGCGCATCATGCGCGGGAACAACCCCATGATTGc                                                                                                                                                                                                                  >  1:245395/1‑251 (MQ=255)             aCCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCg                                                                                                                                                                                                                <  1:218443/250‑1 (MQ=255)                         gCGAGGGGTAACACTTTGTTGCCCGGTAAAATGCTGGCCAGCATTAACATAATGGGAATCAGTAGCAGACCGCCAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCCCGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAacac                                                                                                                                                                                                                           >  1:144610/1‑227 (MQ=255)                             ggggTAACACTTTGTTGCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGTATCAGTACCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcatc                                                                                                                                                                                                  >  1:237658/1‑248 (MQ=255)                                             gCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGATCTTTCTTGCACCGTCGGAGATGTGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAg                                                                                                                                                                                                <  1:161041/234‑1 (MQ=255)                                                                  aaTTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCTCAACCTTTAAAGCCTTCACCAGCCGCCAGGc                                                                                                                                                          <  1:83197/251‑1 (MQ=255)                                                                                                                 gATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGAATCATGCGCGGTAACAACACCATTATTGCCGCAACGGTAATCATCAGACTGGCGCAACCttt                                                                                                                                                                                 <  1:238761/181‑1 (MQ=255)                                                                                                                                                                                                              gcagcaGGCCCTCAACAATCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCATGCAAGGGATTTTTTCATAGATAGCATCAAGTAGAAc                     >  1:8440/1‑244 (MQ=255)                                                                                                                                                                                                                          caacaaTCAGGCGAATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACTCCGATAAGCACCGGATCGCCGGCCATGCCGTGGCGTGTCTGGATCTCCTGAGCATCGATATTGCGTCCCGTCATGAAAGTGATTTTTTCGTAGATAGCATCAAGTAGATCGAAAAGCGGTACTGAACTg  <  1:205468/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              AAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAAAATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCATGAAAGGGATTTTTTCATAGATAGCATCAAGTAGAACGAAAAGCGGTACTGAACTG  >  NC_000913/4529234‑4529701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑