Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,577,700 G→A L87F (CTT→TTT)  mcrC ← Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,577,7000GA92.9% 31.1 / ‑5.4 14L87F (CTT→TTT) mcrCType IV methyl‑directed restriction enzyme EcoKMcrBC subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/9);  minor base C (0/1);  total (4/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAGATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCAT  >  NC_000913/4577457‑4577905
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                             
cacTGAGAACGAATTTCTAATAACGCGGATTTTTGCCGCAATTCAGATAGCGGAAATGCTGCGGAGTTAAATGAAGAGTGCGAAGGACCGGTAATTTTCGATAAAGTGACTGAGCGGCAGCTTTGAGAGTTGAATTTAACTTTTCAGGCTTAATTAATATGGCGAATGGTCTTTTGATAATTCTTGTAGCCAGAGTGTGTTCAGTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAg                                                                                                                                                                                                        <  1:179117/251‑1 (MQ=255)
                              tttttCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACGGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTGTAGCAAACTCTATTCg                                                                                                                                                                           <  1:81945/250‑1 (MQ=255)
                                                   tGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTGCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCgn                                                                                                                                                                          <  1:31991/230‑2 (MQ=255)
                                                                           gagTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAAtcaagc                                                                                                                             <  1:58457/251‑1 (MQ=255)
                                                                                                 tCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCATGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGc                                                                                                        <  1:238792/250‑1 (MQ=255)
                                                                                                        aGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAACATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTc                                                                                                                                                                            <  1:199899/175‑1 (MQ=255)
                                                                                                           gAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAAccc                                                                                              <  1:58076/250‑1 (MQ=255)
                                                                                                              ngAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAAtcaagctcaa                                                                                                                         >  1:64688/2‑220 (MQ=255)
                                                                                                                  cTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAACGTGCTTTTTATAATTAGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGCTTTCCCCTGATTAAAATGGAAGCCGCGTATTGTTTTAGCAATCTCTATTCGACCTTTGATGCCAGGACTGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCtttattt                                                                                      >  1:115221/1‑251 (MQ=255)
                                                                                                                                                       aattaatATGGCTAAGGTGCTTTTTATAATTCGGTTAGCCATCTTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCTTGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTGGCCCTTTGATGCCAGGAATGATCTCGGTGGTAGGAGTGTAATCAAGCTCAATCCCGCGGCGTGAAAGCGGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCgg                                                 <  1:104725/251‑1 (MQ=255)
                                                                                                                                                                                       ggTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTcc                 <  1:12883/251‑1 (MQ=255)
                                                                                                                                                                                            gCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGcc                                                                                                                                                               <  1:87863/104‑1 (MQ=255)
                                                                                                                                                                                                      nttCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGATTAATTTCCTGTAAATAACCCCAt  >  1:53211/2‑251 (MQ=255)
                                                                                                                                                                                                                cATATCAAAAGTACTGACGGTTTTCCCATGATTAAAATGGACGCCGCGTATTGTTTTAGCAAACTCTATCCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTCAAACCCCTTTATTTAATACATACCCCAGGATATCaaga                                                              >  1:116160/1‑181 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                             
CACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTTCATCTCTGATAGTTGAATTTAACTTTTCATGCTTAATTAATATGGCTAATGTGCTTTTTATAATTCGGTTAGCCAGCGTGTCTTCATTAAGCATATCAAAAGTACTGACGGTTTTCCCATGATTAAGATGGAAGCCGCGTATTGTTTTAGCAAACTCTATTCGCCCTTTGATGCCAGGAATGATCTCGGTGTTAGGATTGTAATCAAGCTCAAGCCCTCGGCGTGAAAGCTGTAAAACCCCTTTATTTAATACATACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCAT  >  NC_000913/4577457‑4577905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: