Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	808,338	C→T	G307E (GGG→GAG)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	808,338	0	C	T	92.3%	30.3 / NA	13	G307E (GGG→GAG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/7);  minor base G (0/1);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAGCGCCGCCATATTCACCGGATGAGTGGTTTCGACCACGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGG  >  NC_000913/808119‑808515                                                                                                                                                                                                                            |                                                                                                                                                                                  tccggcgcCGCCATATTCGCCGGATGAGGGGTTTCGACCCGGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCAGCGCGGGCGGGGGCAAGTTGTTCGCGCAGCCGAACTTCAAGAGCCGCCACCTGTTGCTGCCAGTCTCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTAc                                                                                                                                                     <  1:245118/246‑1 (MQ=255)  gCAGCGCCGCCATATTCACCGGATGAGTGGTTTCGACCACGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCttagatcgaag                                                                                                                                                     >  1:1278/1‑240 (MQ=255)   cAGCGCCGCCATATTCACCGGATGAGTGGTTTCGACCACGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATACAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTg                                                                                                                                                   >  1:195943/1‑250 (MQ=255)                               gTTTCGACCACGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCCCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCTCCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAg                                                                                                                      <  1:45099/251‑1 (MQ=255)                                           ccAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGCGGCAAGTTGCTCGCGCAGCTGTACTTCCATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGCTTGCCCATACAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCa                                                                                                                                       >  1:118276/1‑222 (MQ=255)                                                          aCGCGCACATCTGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTGTGCTTCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTgccgn                                                                                            <  1:63614/250‑2 (MQ=255)                                                          aCGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTgccgcc                                                                                           <  1:12746/251‑1 (MQ=255)                                                          aCGCGCACATCGGCAACAATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCCACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGGGAGTGTGGc                                                                                                                >  1:522/1‑230 (MQ=255)                                                                                                                                     aCCTGGTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTTGCGTTTGCTTCCGCGCAGGCCAGCGGATTGCCCATAAAATTTGGCGGATGTATAAAGCAACCCGCTTCACCGTTACTGATGGTTTCTACAACCTCGCGCGTGGTGAGTGTGGCTGAAACGGTCGTTGTGGGGCCGGTTAAGGCGTTTCCGAGGCGCAAAATGTCCGGCGCGATTTCTGCa                                             <  1:131469/222‑1 (MQ=255)                                                                                                                                               gnNAGTCGCNNGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCtt                                                                                                                                                              <  2:1278/99‑1 (MQ=255)                                                                                                                                                   gTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTgg  <  1:70903/251‑1 (MQ=255)                                                                                                                                                      gtcAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCCCCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAg                                                                         <  1:163543/175‑1 (MQ=255)                                                                                                                                                                                      ntgcCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCTAATGCATAAAGCAACCg                                                                                                                                                                  >  1:54712/2‑56 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                  TGCAGCGCCGCCATATTCACCGGATGAGTGGTTTCGACCACGCCAATGGCCCCCAGTACGCGCACATCGGCAACCATTTCGGCATCACGGGCGGGGGCAAGTTGCTCGCGCAGCTGTACTTCAATATCCGCCACCTGTTGCTGCCAGTCGCCAGATTCGAGAATCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGG  >  NC_000913/808119‑808515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑