Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,996	G→A	A29T (GCT→ACT)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,996	0	G	A	100.0%	12.8 / NA	6	A29T (GCT→ACT)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

CCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAAT  >  NC_000913/1881753‑1882209                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      ccGGCGAGCCACCAGCATACAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGACTGCCGc                                                                                                                                                                                                                >  1:214695/1‑251 (MQ=255)                                                                 nttttATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGACTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGCGCAAATGGAGTGGGc                                                                                                                                                                   >  1:157528/2‑232 (MQ=255)                                                                                         gATTAATGCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGAGGGTCAGGAAGTGAGAACACAATGATGAAAGCTATGCGGATTGCTGCGAGCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGTGATTCGCGTGTTACAGACTGCCGCTGAGCGCTGGGGCTTCgtct                                                                                                                                                                                            <  1:36105/183‑4 (MQ=255)                                                                                                                                                         gaNNNNNATGNTGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGACTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGc                                                                                                                                                                   <  2:157528/144‑1 (MQ=255)                                                                                                                                                                                                               naaaGACGTCCTTCCTGAAGGGATTCGCGTGTTACAGACTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAAt  >  1:66722/2‑251 (MQ=255)                                                                                                                                                                                                                                  gggATTCGCGTGTTACAGACTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTgn                                                                         <  1:47030/161‑2 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      CCGGCGAGCCACCAGCATAAAGACGCGCAAATCATTCAGCAGCGGTAAATTATTCATGATTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGATGGTCAGGAAGTGAGAACCCAATGATGAAAACGATGCGTATTGCTGCGATCCCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTTACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAAT  >  NC_000913/1881753‑1882209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑