Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,929,111	G→A	P199P (CCC→CCT)	yebE ←	DUF533 domain‑containing inner membrane protein YebE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,929,111	0	G	A	100.0%	24.6 / NA	10	P199P (CCC→CCT)	yebE	DUF533 domain‑containing inner membrane protein YebE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

ATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATG  >  NC_000913/1928894‑1929279                                                                                                                                                                                                                          |                                                                                                                                                                         aTGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATAACGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCTAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTCTTTGCTGTTCGAGATCGCTTTCAATGCCGTCACGAACATGCTGAGGGATTTTCAGCGCGTCACCCAGTGCAGTCAgn                                                                                                                                         <  1:52111/251‑2 (MQ=255)                    gTCATTGCTTAGATGCTGACAGGTACAGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCATGTTCAACTGGCTGTGCCATTCTGGACAGCCCGCAATCGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGACAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGCCCATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAAt                                                                                                                      >  1:100603/1‑251 (MQ=255)                                                            aaTGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTc                                                                              >  1:121284/1‑251 (MQ=255)                                                                                 aTCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCTTCTGCTTCATTGCg                                                         <  1:57598/251‑1 (MQ=255)                                                                                                                                        ggCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGTAACTCAGTAAATAGATTTCCAGCGCCGCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAAtn  <  1:25997/251‑2 (MQ=255)                                                                                                                                               cAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCa     >  1:237131/1‑241 (MQ=255)                                                                                                                                                      cGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATACTGAGGGATTTTCAGAGCGTCACCCAGTGCATTCAGGTAACTGCGtt                                                                                                                               >  1:176894/1‑112 (MQ=255)                                                                                                                                                      cGGAATTATTCCGCCAAAGTGCGNTTTNGCTGNTCNAGATCGCGTTCAATGNCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCANNCNNNTNACTGCGtt                                                                                                                               <  2:176894/112‑1 (MQ=255)                                                                                                                                                                           cGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGAGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCg                                                         <  1:62116/161‑1 (MQ=255)                                                                                                                                                                           cGTTTTTGCTGTGCGAGATCGCGGTCNATGGCGNNGCNAACATCCTGAGGGATTTTCNGCNNNTNNNCNGGTGCNTTNNGGTACCTGCNTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCANCGCCTCTTCNNCATTGCg                                                         >  2:62116/1‑161 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                         ATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGATATCGAATGTTATGCAGACAGAGAAAATCAGCCTGTTCAAATGGCTGTGCGATTCTGGATAGCCCGAAATAGTCAACTTCAGGCTATCCAGAGAGCGGAATTATTCCGCCAAAGTGCGTTTTTGCTGTTCGAGATCGCGTTCAATGCCGTCACGAACATCCTGGGGGATTTTCAGCGCGTCACCCAGTGCATTCAGGTAACTGCGTTCCATAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATG  >  NC_000913/1928894‑1929279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑