Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,955	C→T	intergenic (‑5/‑115)	ybiA ← / → dinG	N‑glycosidase YbiA/ATP‑dependent DNA helicase DinG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,955	0	C	T	100.0%	20.9 / NA	10	intergenic (‑5/‑115)	ybiA/dinG	N‑glycosidase YbiA/ATP‑dependent DNA helicase DinG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

GGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCG  >  NC_000913/832740‑833156                                                                                                                                                                                                                        |                                                                                                                                                                                                          gggAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAACTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGc                                                                                                                                                                                                        >  1:34433/1‑219 (MQ=255)                                                                       tGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAg                                                                                                                                                                                 <  1:21516/172‑1 (MQ=255)                                                                                 cAGGCTTTCCCGTCAACCTTGATGGGCCAGGCGGCCAAGTTAGAAAAATGCCAGTAGTCCTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTCAATCGCATTATGTTGGTGGTTATTGCGCGCCGCTTTCCAGAAACAGACAAACCATTACCACTGAAAACCGAAAAATGCAACAATCTTGGATGTTTATAAAGTATTTCAGGTTTtctc                                                                                         >  1:167415/1‑250 (MQ=255)                                                                                                          nccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGCGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATACTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAgc                                                               >  1:48248/2‑251 (MQ=255)                                                                                                              agcggcAAAGATAGAAAAATCAACGTAGTAAGCGCTGGTGATGTAGAAATATATGAAGGTGTCTTGTATGACGAGTTGGAGCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGAGGATATTGCGAGACGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGATGTTTATACAGTATTTCAGGTTTTCTCATGGTATTAACCGCAGCGCTTAAAGCGCa                                                            >  1:240045/2‑250 (MQ=255)                                                                                                                   aaaaGTTAGAAAAATCACCGGAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTgn                                                      <  1:204032/250‑2 (MQ=255)                                                                                                                                   aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCGCAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGgcg                                      <  1:44330/251‑1 (MQ=255)                                                                                                                                                                       ggTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCCTTATGTTGGTGGTTATTGCGAGCAGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACCGATCCCCGACTTTATTCCCCGTgcg  >  1:245564/1‑251 (MQ=255)                                                                                                                                                                       ggTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTGGGTGGTTATGGCGAGCCGCTTTCCAGAAACAGAAAAACCATTCCCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTgc   <  1:60383/250‑1 (MQ=255)                                                                                                                                                                                                    gtgCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAAAAGAAAACACATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGTCGCGCt                                                                     >  1:242520/1‑155 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                          GGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCG  >  NC_000913/832740‑833156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑