Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,796	C→T	pseudogene (33/427 nt)	insN →	IS911A regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,796	0	C	T	100.0%	18.1 / NA	9	pseudogene (33/427 nt)	insN	IS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

GAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCG  >  NC_000913/271556‑272023                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    gagTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCg                                                                                                                                                                                                                           <  1:81201/251‑1 (MQ=255)                           agaATACAAATGGGCTAATTCGGTAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATTCGCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGAGAGTTGAAAGGGGGGTTGCATTGACAGATTGAATCTACAACCGCTCTCTTGAGGTCAGACTCCCTGAATAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCtgtn                                                                                                                                                                                                 <  1:26141/251‑2 (MQ=17)                                      gggCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTGCAAAACACCTAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGc                                                                                                                                                                                               <  1:209932/242‑1 (MQ=255)                                       ggCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGAAACTCTGGGCGCGTTATCCTGTGGc                                                                                                                                                                                               <  1:193646/241‑1 (MQ=255)                                                                                           acatgaacatgaTCTGGGTGCTGCTCAGCTAAACAACAGACCAAGAAAGACCCGTAAGTTCAAACCACCGAAAGAGATAATTGAAAGGGGTGTTGCTTTGACAGATGGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTGCGATATTCGGGAATCTCAGGTGGCTTTATCCTTTGGCCCCTCTCTTCATTGTGTTCGGGGTCCATCGCGACAGCTACAAATACTGGAAAAACCGTCCTg                                                                                                                                 <  1:229276/251‑1 (MQ=16)                                                                                                                                                                                      gtTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCAGGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGc                                      <  1:235638/251‑1 (MQ=255)                                                                                                                                                                                              gACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCAt                                                                            >  1:230891/1‑205 (MQ=255)                                                                                                                                                                                                                          gATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTAATCGCAGCAGCTACAAATACTGGAAACACCGACCTGAAAAGCAAGAAGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGACAACCCAGAGAGGATACCAGATGGGgcg  >  1:189759/1‑251 (MQ=37)                                                                                                                                                                                                                                          aaCAGTTTTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTGTCTGCCATGTGTTCGGGGTTCATCGCCGCAGCTACGAATACTGGAACAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCcac                                   >  1:241326/1‑202 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    GAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCG  >  NC_000913/271556‑272023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑