Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,397	G→A	A43V (GCT→GTT)	ydeN ←	putative sulfatase YdeN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,397	0	G	A	88.9%	13.6 / ‑3.3	9	A43V (GCT→GTT)	ydeN	putative sulfatase YdeN
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (2/6);  total (2/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

TTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAG  >  NC_000913/1582240‑1582581                                                                                                                                                              |                                                                                                                                                                                         tttGTAGGTATCGACAACTTCTCGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCAGGGTCAGTACGATTATATTTGGCTTTCCTTTGGGACGGTATTCTGTCGGCGTAGAGTCTGAAAAAGCAACGGTTGGTTTGTTTGCGTTCTGCGGTAGATCATCGGCCGCATGAGCAGCAAGTGCAGCCATACCAGATGCCAGTATCAAAGATATCGAg                                                                                              <  1:127608/250‑1 (MQ=255)                       cGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATAGTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGGCTGGTAAAACAACGTTTGGTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCATCAAATGCAGcc                                                                                                                            <  1:35461/198‑1 (MQ=255)                           tttCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGc                                                                    <  1:8222/250‑1 (MQ=255)                                   gTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGNTTTNNNNNCtnn                                                                                                                                                                    >  2:154674/1‑144 (MQ=255)                                          ngTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGtt                                                    >  1:65180/2‑251 (MQ=255)                                                                          ccATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATg                                                                                                                                            <  1:3159/131‑1 (MQ=255)                                                                                   aGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCAGCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTGACGACACTTTTCCGTAATGCAGACTTCTTAAATGTTCCTGTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAag           <  1:165638/251‑1 (MQ=255)                                                                                       catcCATGGTCAGGACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTGCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGa             <  1:212055/245‑1 (MQ=255)                                                                                            aTGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAACAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGATGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAAATCCAAAGTAATTTTACGAAGAGAAATaan  <  1:154674/251‑2 (MQ=255)                                                                                                                                                              |                                                                                                                                                                                         TTTGTAGGTATCGACAACTTCACGATTTTCCATTGTTTTTGGGTCAAAAGATCCCTTATCAAAAGGAAGTTGTCCATAACCAAGATCATCCATGGTCAGTACGATAATATTTGGCTTTCCTTTGGTACTGTATTCTGTCGGCGTAAAGTCTGAGAAAGCAACGTTTGTTTTGGTTGCTTTCAGCTTTACATCATCTGCCGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAG  >  NC_000913/1582240‑1582581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑