Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,288	C→T	intergenic (‑163/‑3)	yaeP ← / → yaeQ	PF06786 family protein YaeP/uncharacterized protein YaeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,288	0	C	T	100.0%	26.6 / NA	10	intergenic (‑163/‑3)	yaeP/yaeQ	PF06786 family protein YaeP/uncharacterized protein YaeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

GCCCAGCGCGTCCGGAACGTATCCTAAGTCTCCGCTGGCGATTTCCGCGTACCGCTTGCGTATTAACTCACAATATTTTTCCACATGCCCTCCTGTCAGCACTCTGACTTAACCGTGGATGCAAGTCTAAGCCTACGAAGATAAACTCTGTTTCGCAAGGTGACTATACCACACTCATTTCTGCAATATCAGCGCCGCAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGG  >  NC_000913/214042‑214520                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         gCCCAGCGCGTCCGGAACGTATCCTAAGTCTCCGCTGGCGATTTCCGCGTACCGCTTGCGTATTAACTCACAATATTTTTCCACATGCCCTCCTGTCAGCACTCTGACTTAACCGTGGATGCAAGTCTAAGCCTACGAAGATAAACTCTGTTTCGCAAGGTGACTATACCACACTCATTTCTGCAATATCAGCGCCGCAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTTTTAt                                                                                                                                                                                                                                      >  1:120455/1‑251 (MQ=255)                                                                                        cncTCCTGTCAGCACTCTGACTTAACCGTGGATGCAAGTCTAAGCCTACGAAGATAAACTCTGTTTCGCAAGGTGACTATACCACACTCATTTCTGCAATATCAGCGCCGCAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTTTTATGGCGCTTa                                                                                                                                                                                                                              >  1:199701/1‑172 (MQ=255)                                                                                         cctcctGTCAGCACTCTGACTTAACCGTGGATGCAAGTCTAAGCCTACGAAGCTAACCTATGTTTCGCAAGGTGACTATACCACACTCCTTTCTGCAATATAAGCGCCGCAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGACAGGAGTTTTTTTATGGCGCTTAAAGCGACACTTTATAAAGCGACGGTTAATGTGGCCGAtct                                                                                                                                                                                      >  1:135665/1‑211 (MQ=255)                                                                                                                                                            cAAGGTGACTATACCACACTCATTTCTGCAATATCAGCGCCGCAACTGCACGTATTCCGTTCCACTGGCCTCCTGATTCGACAGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCACCATCCTTCAGAAACCCAGGAGCg                                                                                                                    >  1:235374/1‑210 (MQ=255)                                                                                                                                                                                                 cgccgcAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTTGTTTGTGTGCCgatga                                      <  1:18570/251‑1 (MQ=255)                                                                                                                                                                                                           gCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAA‑TATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGa                             >  1:99740/1‑249 (MQ=255)                                                                                                                                                                                                                              ggCCTCCTGATTCGAAAGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGTCGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGAtn         <  1:28731/251‑2 (MQ=255)                                                                                                                                                                                                                                   cctGATTCGAAAGGAGTTTTTTTATGGCGCTTAAAGCGCCAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAACATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGACGAGTGGCTGCgcgctaccagcgcg    >  1:39658/1‑238 (MQ=255)                                                                                                                                                                                                                                     ngATTCGAAAGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTggg  >  1:153592/2‑251 (MQ=255)                                                                                                                                                                                                                                           naaaGGAGTTTTTTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGAtc                                                                                                                                                                                       >  1:53229/2‑64 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GCCCAGCGCGTCCGGAACGTATCCTAAGTCTCCGCTGGCGATTTCCGCGTACCGCTTGCGTATTAACTCACAATATTTTTCCACATGCCCTCCTGTCAGCACTCTGACTTAACCGTGGATGCAAGTCTAAGCCTACGAAGATAAACTCTGTTTCGCAAGGTGACTATACCACACTCATTTCTGCAATATCAGCGCCGCAACTGCACGTATTCCGTTACAATGGCCTCCTGATTCGAAAGGAGTTTTCTTATGGCGCTTAAAGCGACAATTTATAAAGCGACGGTTAATGTGGCCGATCTCGACCGCAACCAGTTTCTCGATGCCTCTCTGACGCTGGCGCGCCATCCTTCAGAAACCCAGGAGCGTATGATGCTGCGCTTGCTGGCGTGGCTGAAATATGCTGATGAACGTCTGCAATTTACCCGTGGTTTGTGTGCCGATGATGAGCCGGAAGCGTGGCTGCGTAACGATCACCTGGG  >  NC_000913/214042‑214520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑