Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,712,609	G→A	G52G (GGC→GGT)	yfiC ←	tRNA m(6)A37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,712,609	0	G	A	100.0%	36.6 / NA	14	G52G (GGC→GGT)	yfiC	tRNA m(6)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

GGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACC  >  NC_000913/2712397‑2712817                                                                                                                                                                                                                     |                                                                                                                                                                                                                 gggTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTg                                                                                                                                                                             <  1:123208/250‑1 (MQ=255)                    cGAAGAGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAAt                                                                                                                                                          >  1:195946/1‑250 (MQ=255)                                      ntgtgATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTc                                                                                                                                       >  1:149310/2‑251 (MQ=255)                                                   ctgAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAgac                                                                                                                                                                                    <  1:239151/193‑3 (MQ=255)                                                             gccgTATGGAAGTTAATCCGCTCTGCCTACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCCACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGcaac                                                                                                                >  1:212329/1‑251 (MQ=255)                                                                                gCTCTGCCCACGGGGACTGGTTGATATTTGCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCATCCCGCGACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATTCCATCCGTTCCCACTTTCAGCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTa                                                                                             <  1:238465/251‑1 (MQ=255)                                                                                      cccACGGGGACTGGTTGATATTTTCCTGCGCCTGCGAGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTc                                                                                                                                       >  1:58545/1‑203 (MQ=255)                                                                                                ntanttGNTNNTNNCCTNCGCCTGCGCGGCAGCTTCACTNNCCAGTTCAANTGCATCAATCATCACGCTGTCATCCGTTCGCTGCGCCAGCATTAATGCCAGCANCCCGCTACCNNCANCGATANCNNNGNNNCTNTTTTCCCCAGCCACCGTTGNCCNNGCGCCCNATAAAATACCATCCGTTCCCACttgc                                                                                                                                       <  2:58545/189‑3 (MQ=255)                                                                                                                                                     cATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTc                                                                                                                                               <  1:211189/132‑1 (MQ=255)                                                                                                                                                                           ggTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCCACCCGAACCGATATCAAGGCAACGTTTTACCCCAGCCCCCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCAAAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGGGAGAACAATAcc  >  1:83362/1‑251 (MQ=255)                                                                                                                                                                            gTTCGCTGCGCCAGCATTAATGCCAGCAACCCGTTACCCGCACCGATGACAAGGAAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATCCCATCCGTTCCCACTTTAATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACACGAAAATAAAACCTTGCAGGAAAAACGGCg                          <  1:22253/226‑1 (MQ=255)                                                                                                                                                                            gTTCGCTGCACCAGAATTAATGCCAGCAACCCGCTCCCCGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGcaac                                                                                                                >  1:201983/1‑140 (MQ=255)                                                                                                                                                                                                        aCNNNNNACCNGCACCGAGATCAAGGCAACGTTTTCCCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTacgac                                                                                <  2:149310/144‑1 (MQ=255)                                                                                                                                                                                                                cccGCACCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGAACGGATGTAGACTGTGACATGAAAATAAAAc                                            >  1:137126/1‑172 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                 GGGTTACTGATGATTAAATCGAAGCGTACTGTCTGCTGTGTGATCCACTGCTGAATATCCGCCGTATGGACGTTAATCCGCTCTGCCCACGGGGACTGGTTGATATTTTCCTGCGCCTGCGCGGCAGCTTCACTTTCCAGTTCAACTGCATCAATCATCACGCTGTCATCGGTTCGCTGCGCCAGCATTAATGCCAGCAACCCGCTACCCGCGCCGATATCAAGGCAACGTTTTACCCCAGCCACCGGTGCCCATGCGCCCAATAAAATACCATCCGTTCCCACTTTCATCGCACAGCGATCGTGAGCAACAAAAAACTGTTTAAAAGTAAATCCATTACGACGAAGCACGGATGTAGACTGTGACATGAAAATAAAACCTTGCAGGAAAAACGGCGATAGCACCGGGTGAGAACAATACC  >  NC_000913/2712397‑2712817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑