Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,500	G→A	L252L (TTG→TTA)	pheA →	fused chorismate mutase/prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,500	0	G	A	100.0%	29.5 / NA	12	L252L (TTG→TTA)	pheA	fused chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

TGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCC  >  NC_000913/2738287‑2738723                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 tgGCGAGATGACGTTAACTATCGAACATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCAAAAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCCTGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAgg                                                                                                                                                                                             >  1:92097/1‑251 (MQ=255)                             tgtttgttGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAgn                                                                                                                                                                  <  1:50432/250‑2 (MQ=255)                                          ccGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTCCTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCAcc                                                                                                                                                    >  1:182730/1‑251 (MQ=255)                                                          aTCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCCAATTACTCAATCGTTATCCGCACTGGAAGATTGAATATACAGCAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGCAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCCGATTTGTGGTGTTg                                                                                                                                    >  1:137822/1‑251 (MQ=255)                                                                                           gCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGGCTGCGGCAATGGAAAAGGTTGCTCAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTgg                                                                                                                                                                                       <  1:235396/167‑1 (MQ=255)                                                                                             naTTCCAGCAATGCAGCAAATTCCTTACTCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCg                                                                                                >  1:64997/2‑251 (MQ=255)                                                                                                                                                           aGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTAc                                                                                                                                                                                                       >  1:110465/1‑87 (MQ=255)                                                                                                                                                                       gCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATgg                                                                          <  1:218518/199‑1 (MQ=255)                                                                                                                                                                       gCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCa                       >  1:115525/1‑250 (MQ=255)                                                                                                                                                                          aTGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCCAATCAGCGAAAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACACGCCGGTGCGCTGGTTGAAGCGTTGCTGGt                               >  1:110597/1‑239 (MQ=255)                                                                                                                                                                                        acaGGCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGTAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAATCCACGTTGTTAATGGCGACCGGGCAACAAGCCgg                                                       <  1:200398/201‑1 (MQ=255)                                                                                                                                                                                           ggCAAAATCACCGCATGTTGCTGCGTTAGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGatcc  >  1:96595/1‑248 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                 TGGCGAGATGACGTTAACTATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCCAGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCC‑GATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCC  >  NC_000913/2738287‑2738723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑