Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,106	G→A	L222F (CTC→TTC)	abrB ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,106	0	G	A	100.0%	13.4 / NA	7	L222F (CTC→TTC)	abrB	putative regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

AAGCCCGCCGGGGCTGGTGGCGAGGTAGGCAGTCATAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCC  >  NC_000913/746870‑747316                                                                                                                                                                                                                                             |                                                                                                                                                                                                                   aggCCCGACGGGTCTGGGGGCGAGGTAGGCAGTCATAAAACAAATGTGCATAACCCGGGTCAGCCCCCACGCCATACCCGCACAGATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTGGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGCCCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGGTCGAGGGTGATGAACTGACCAGACTGGa                                                                                                                                                                                                      <  1:12753/249‑1 (MQ=255)           gggCTGGTGGCGAGGTAGGCAGTAATAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCATGAGTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCATCAGCCATTCCGGTAGTTCGATGGTGATGAACTGACGAGTCTGGAGCACTGcgcc                                                                                                                                                                                            <  1:94683/251‑1 (MQ=255)                                            aTGTGCAAAACCCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGAAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACAAATCCGCGAGCCAATTGCCATATCCGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAACTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCggaagg                                                                                                                                                           <  1:81590/251‑1 (MQ=255)                                                                          aTACCCGCACAAATAGCGAGCAGAGCAAAAATCGACAGCAGGATTGGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTTTTTTTCGAAACCAAGACCAATCCTCCAGCCAATTGCCATAGACGCCATCGCCAGCAGCCATGCCGGTAGTTCGATGGTGATAAACGGACCAGCCTGGAGCGCTGCGCTCGCCAGCAGCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCGCTGTACCGGc                                                                                                                             <  1:187039/251‑1 (MQ=255)                                                                                                                                          cccGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATCCGCCATCGCCAGCAGCCACTCCGGTAGTTCGATGGTGATGAACTGACCAGACTGGAGCACTGCGCTCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGCAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCaaacattatgt                                                             >  1:147801/1‑241 (MQ=255)                                                                                                                                                                                                     gNCATCGACAGCAGCCATTCCGGTAGTTCGATGGTGATGAACTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATTCTGGTTAACCGCTTCCGCGTTATCCCCCAGCACCATGCGGGGGACCCGAACCGCGGcccc  >  1:198761/1‑251 (MQ=255)                                                                                                                                                                                                           gCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAACTGCCCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGAATCGCGCCGGAAGGAAGACGCAGCAGGCATCCCCCTGTACCGGCAACGAc                                                                                                                       >  1:187582/1‑128 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                   AAGCCCGCCGGGGCTGGTGGCGAGGTAGGCAGTCATAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGACGCAGCAGGCATCCCACTGTACCGGCAACGACCGCGAGCAAAATAGTAAGCAGGAGATTAATGCTCACTGGCGGGAACCAGACGATATGCTGGTTAACCGCTTCAGCGTTATCGCCCAGCATCATGCGGGTGACCAGAACAGCGGCCCC  >  NC_000913/746870‑747316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑