Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,617,368	G→A	D16N (GAC→AAC)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,617,368	0	G	A	100.0%	32.5 / NA	13	D16N (GAC→AAC)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

GAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTA  >  NC_000913/4617165‑4617560                                                                                                                                                                                                            |                                                                                                                                                                                                 gAAAGTGAATTATTTGAACCAGATCGGATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGATGTGTGTTGGGGAGTAAATGTTAGAATACGAACAAACTCGCAAGGTAAATTTTATTGGCGACAAGCCAGGAGAATGAAATGAGTGATCTGAAAGCAATCAGCTTGCGTGCACTAAAAGTGAGGAACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGcc                                                                                                                                                   <  1:121658/251‑1 (MQ=255)                          gCATTACAGTGCTGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATAGACGTGTGTTGCGGAGTAAATGTTAGAATACTAACAATCTCGCCAGGTGAATTTTATTGGCGACACGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGCCGACACcgac                                                                                                                                                                  >  1:175309/1‑211 (MQ=255)                                                              tAATTGGGATGTGTATCAAAGTGTGTTGCGGAGTAAATGTTAGAATATTAACAAACTCGCAAGGTGAATTTTATGGGGGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCACCCTGCGGGCACTGAAAGTGATGACCCTGACCCCCCGGAATGACGACAACACCGGCAAGAAAGTGATCGCCCTGGGTCATCAGGCCAAAACTGCGGCCGGCAATACCGCCGCTATCTGTATCTATCCTCGc                                                                                       <  1:38518/250‑1 (MQ=255)                                                                                                  aTGTTAAAATACTAACAAACTCGCAAGGTGAATTTTATTGGAGACAGGGCAGGAAAATGAAATGACTAATCAGAAAACAAGCAGCCTGCGTGCACTGAAATTGATGAGCCTGACCACCCTGAATGGCGAAGACACCGACGAGAAAGTGATCGCCCTGTTTCATCAGGCAAAAACTCCGGTCGACATTACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAgg                                                  <  1:212196/251‑1 (MQ=255)                                                                                                   tgttagaaacctaaaaaccccGCAAGGGTAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCATCCTGCGTGCACTAAAATTGATTACCCTGCCCCCCCTGAATGACGACGACACCGACAAGAAAGTGATCGCCCTGTGTCATCAGACAAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGan                                                                            <  1:159463/204‑2 (MQ=255)                                                                                                   tGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGACGACANCGANNNNna                                                                                                                                                             >  2:159463/1‑143 (MQ=255)                                                                                                              tAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGACGACACCGACGAGAAANTGann                                                                                                                                                      >  2:152673/1‑137 (MQ=255)                                                                                                              tAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGAtn                                                                                                                                                      <  1:152673/139‑2 (MQ=255)                                                                                                                            ngTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACg                        >  1:150717/2‑251 (MQ=255)                                                                                                                             gTGAATTTTATTGGCGACAAGCAAGGCGAAAGAAATGACTGCTCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACATGACAACCCTGAATGACGACGACACCGACGAGAAAGTGATAGCCCTGTGTCATCAGGCCAAAACTCCGGACGGCAATAcc                                                                                                               >  1:75680/1‑163 (MQ=255)                                                                                                                                                   cAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGCAATTGAGGAACCTCACCACCCTGAATGACGACGACAGCGACGAGAACGTGATCGCCCTGTGGCATCAGGCCCAACCTCAGGACGGCAATCCCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGAACCCCGGAAATCCGTCTCGCTACGGTAACCACCTTCCCACACGGTa  >  1:181866/1‑250 (MQ=255)                                                                                                                                                                   gtgcgctgtaagcaggcAGCCTGCGTGCACTGAAATTGATGAATCTTAACACCCTGAATGACGACGACACCGTCGAGAAATGGATCGCNNTGTGtcnnc                                                                                                                                         >  2:15630/10‑99 (MQ=255)                                                                                                                                                                        cTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGAACCTGACCACCCTGAATGACGACGACACCGACGAGCAAGTGAt                                                                                                                                                       >  1:40745/1‑80 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                 GAAAGTGAATTATTTGAACCAGATCGCATTACAGTGATGCAAACTTGTAAGTAGATTTCCTTAATTGTGATGTGTATCGAAGTGTGTTGCGGAGTAGATGTTAGAATACTAACAAACTCGCAAGGTGAATTTTATTGGCGACAAGCCAGGAGAATGAAATGACTGATCTGAAAGCAAGCAGCCTGCGTGCACTGAAATTGATGGACCTGACCACCCTGAATGACGACGACACCGACGAGAAAGTGATCGCCCTGTGTCATCAGGCCAAAACTCCGGTCGGCAATACCGCCGCTATCTGTATCTATCCTCGCTTTATCCCGATTGCTCGCAAAACTCTGAAAGAGCAGGGCACCCCGGAAATCCGTATCGCTACGGTAACCAACTTCCCACACGGTA  >  NC_000913/4617165‑4617560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑