Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,119	G→A	R70H (CGC→CAC)	yhaI →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,119	0	G	A	100.0%	14.2 / NA	8	R70H (CGC→CAC)	yhaI	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCA‑ATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAA  >  NC_000913/3252872‑3253333                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        gAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTCTTTGTCAGTCTTAAAAACTTATGTTGGTTTCTCTGGTCGCGAACGTCGTAAAGAGTACTGGATGTTTACTCTGCTTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACtt                                                                                                                                                                                                                      >  1:145692/1‑251 (MQ=255)                aCTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTACACGATaccgaccg                                                                                                                                                                                                       >  1:208762/1‑251 (MQ=255)                                                                                                   tAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGACATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACt                                                                                                                     <  1:8857/250‑1 (MQ=255)                                                                                                            cTGGATGTTTACTCTGATTCACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTc                                                                                                                                               >  1:96975/1‑215 (MQ=255)                                                                                                                               aaCGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGTTGGCAACCGTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTACACGATACCGACCGTGCAGGTGCCTGGGTCTTGCTCTTTTTTGTTCCGTTTAGTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCA‑ATCGTTACGGAAACGATc                                                                                        <  1:204923/250‑1 (MQ=255)                                                                                                                                                                             ttCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGAAACCTTCCATCCAGACCTTGAGCTTGCCATCCGTCACTAACACGATCACGACCGTTCAGAAGACTGGGCCTTGCACTTTTTTGTTCCGTTTATTAGCTGGGTCGAGCTTCTGGTTTTTTTATGAACAGAAGGTACTTCTGGCAGCA‑ATCGTTAAGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGAGATATTTCGccc                                         >  1:45288/2‑251 (MQ=255)                                                                                                                                                                                                       cTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCA‑ATCGTTACGGAAACGATcn                                                                                       <  1:69409/179‑2 (MQ=255)                                                                                                                                                                                                                     aaCCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCACTTATACGATACAGACCGTTACGGCGCCTGGGCGTTGCTCTTTTTTGTTACGTTTATTGGCTGGCGCGCGCTTCTGGTTTTTTTATGCACAGAAGGTACTTCTGGAAGCACAT‑GTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaa  >  1:245197/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        GAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGCTATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCA‑ATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAA  >  NC_000913/3252872‑3253333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑