Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,569,134	C→T	G65G (GGG→GGA)	glgC ←	glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,569,134	0	C	T	90.9%	22.9 / ‑6.0	12	G65G (GGG→GGA)	glgC	glucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGTATTCCGCTTTATAACGGCGGATAATGTCGAGGTTTTGGGTGACCGCATCTGCGGTGCCGCGATACCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCAGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTT  >  NC_000913/3568920‑3569342                                                                                                                                                                                                                       |                                                                                                                                                                                                                 ccacGTATTCCGCTTTATAACGGCGCATAATGTAGAGGTTTTGGGTGACCGCATCTGCGGTGCCGCGATCCCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCCGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGAt                                                                                                                                                                               >  1:84575/1‑250 (MQ=255)        ttCCGCTTTATAACGGCGGATAATGTCGAGGTTTTGGGTGACCGCATCTGCGGTGCCGCGATACCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCAGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGTGTTATCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCgg                                                                                                                                                                        <  1:190570/250‑1 (MQ=255)                                            gttGACCGCATCTGCGGTGCCGCGATACCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCAGCAGATCGACCAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGTTTCCGGAGTTGATGCAGTTAGACAGCGCAAAGt                                                                                                                                                                                   >  1:167675/3‑203 (MQ=255)                                                              gCCGCGATACCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCAGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGCGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAGCCAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGcc                                                                                                                                                >  1:229416/1‑220 (MQ=255)                                                                                               ntgtgctgGCAGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGAt                                                                                                                                                                               >  1:68501/2‑156 (MQ=255)                                                                                                                 gACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACGTGGTGATCACGCCCATACGACGGATTCCGGAGTTTATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCt                                                                                                                                   <  1:125751/182‑1 (MQ=255)                                                                                                                                         gaagaaTGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGTTGGCGCGCCAACATTAAGTGAt                                      >  1:57894/1‑251 (MQ=255)                                                                                                                                           ngaaTGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTGCTGGGTGATCACGCCCATCCGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATg                                                                 >  1:24017/2‑222 (MQ=255)                                                                                                                                                   cgcccccgctaatggggttgcgccAGAGTTGGGGACTGGTTCTGGTTCATCACGCCCATACGACGGATGCCGAATTTGATGCAGTTAGTCGGCGCAAAGTCGATAATGGGGACCTTACCGCCGATGTGTCCGGCCGGTTTTGCTCGCTAATTGTTTAAATCCGTCAGGGGGCTACCACGCCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGTTGGCGCGCCAACATTAAGTGATCGTTCTtctc                            <  1:113442/229‑1 (MQ=255)                                                                                                                                                                    ctgcACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCCTACGACGGATTCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCg                                                                                                                                     >  1:230680/1‑129 (MQ=255)                                                                                                                                                                            gagTGTGGGACGGGTCCGGGGTGTTCACGCCAATACGAGGGTTCCGGGAGTTGTAGCAGTTAGACAGCGAAAAGCGGATAAGGCGGAACTTCCCGCCGAAGTGTACGTCCGGTTTGGCTCGCTTAGGGGGAAAACCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGTTGGCGCGcc                                                   <  1:245686/203‑1 (MQ=255)                                                                                                                                                                                                   aTCACGCCCATACGACGGATTCCGGAGTTGATGCAGTTAAACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGCAGTGAAAGGCCGGATTTGATCGCTTATTGGTTACATCCTTCAGGCGGGTAACACGTCCTCCCGCCAGCATCAGGGCAACAGATTTTAATGGCAGCTGGCGCGCCAACCTTAAATGTTCGTTCTTCTCTAAACTAACCATGACTAACTCCtttt  >  1:226037/1‑229 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                 CCACGTATTCCGCTTTATAACGGCGGATAATGTCGAGGTTTTGGGTGACCGCATCTGCGGTGCCGCGATACCAGTTTTCCCCTTTCATTCTCTGCTGTGCTGGCAGCAGATCGACAAACTCGTTCATTTCTTCATTGAAGAATGACCAGCCGCGCTGAATGTGCTGCACCAGAGTGTGGGACTGGTACTGGGTGATCACGCCCATACGACGGATCCCGGAGTTGATGCAGTTAGACAGCGCAAAGTCGATAATGCGGAACTTACCGCCGAAGTGTACGGCCGGTTTTGCTCGCTTATTGGTTAAATCCTTCAGGCGGGTACCACGTCCTCCCGCCAGTATCAGGGCAACAGATTTCAATGGCAGCTGGCGCGCCAACATTAAGTGATCGTTCTTCTCTAAACTAACCATGACTAACTCCTTTT  >  NC_000913/3568920‑3569342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑