Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,602,252	G→A	L203L (CTG→TTG)	yjjP ←	putative succinate exporter YjjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,602,252	0	G	A	100.0%	24.1 / NA	10	L203L (CTG→TTG)	yjjP	putative succinate exporter YjjP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

TGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATG  >  NC_000913/4602013‑4602433                                                                                                                                                                                                                                                |                                                                                                                                                                                      tGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAACAGAACGCTg                                                                                                                                                                             <  1:187335/250‑1 (MQ=255)     cacCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCCACGCTAACAGAAATTCAATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGCTGTGGCCTTTAAACATCTCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGAAACAGAACGCTggcggc                                                                                                                                                                        >  1:115153/1‑251 (MQ=255)                                                           cGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAACAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCgg                                                                                                                   >  1:74934/1‑250 (MQ=255)                                                                   gAAATTCGATCACACCCATCCGCGTAGCCCCCCAATCGCCAGTGCCATCCCTCAGCCGACGCCGGTAGACAGCGTCAGCAGCCTGGCGCCCGCCAAGAGTGCCAGTCCGGTAATCATGTGGCCTTTAAACATATAGGCGACGGCATTAATCAACGGAAAGCCCGGCCCTAGCAACAGACCGCCGGCGGCCATCGCAATGGCGGGGGTATTGCAGAACGAGGGGAGTTGCAAAAGCAATCCCGAAAtggtgg                                                                                                          >  1:236329/1‑251 (MQ=255)                                                                                                                                       ccAGTGTCAGCAGACTGGCGAGCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACGAGCAACAGAACGCTGGCGGCCATCGCAATGGTggggg                                                                                                                                                       <  1:229932/138‑1 (MQ=255)                                                                                                                                        cAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAACAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCagcanc                                            <  1:197878/244‑1 (MQ=255)                                                                                                                                                                       gCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAACAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGGGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGAGGACGTTGTGCCAGCAGCTTGCGGATATACATCGCGGTCGTACTGGCAAAGAAgg       <  1:225070/250‑1 (MQ=255)                                                                                                                                                                           gTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGCAAGCCCGGCACTAGCCACAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAACGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGCGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGACGGtgatg  >  1:248057/1‑251 (MQ=255)                                                                                                                                                                               tgTATGGATGTGGTCTTTTAACATATGGGCCACGGCATGAATCAACGGAAAGCTTGGTGCTTGCAACAAATCGCTGGCGGCCATCGCAGTTGTGGGGGGATTTCTCAAAGCGGGGAGTTGCTGAAGAAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAgg                                                                                      <  1:122829/162‑1 (MQ=255)                                                                                                                                                                                           gCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAACAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGcc                                                  <  1:20827/187‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                      TGAACACCATCGCAAAGCCGACCGCAGGAATGGCGGCGAGGATCATATCCTGCGCCAACGCTAACAGAAATTCGATCACACCCATCCGCGTAGCCCCCAAATCGTCAGTGCCATCACTACGCCGACGCAGGTAGCCAGTGTCAGCAGACTGGCGATCGCCCAGCGTGCCAGTCCGGTATTGATGTGGCCTTTAAACATATCGGCGACGGCATTAATCAACGGAAAGCCCGGCACTAGCAGCAGAACGCTGGCGGCCATCGCAATGGTGGGGGTATTGCTGAAAGTGGGGAGTTGCAAAAGCAATCCGGAAATGGTGGTGGCGGCGAAAGCGGTAAGGCAAAAGTTGATCTGTGGATGAAGATGACGTTGTGCCAGCAGCTGGCGGATATACATCGCGGTCGTACTGGCAAAGAAGGTGATG  >  NC_000913/4602013‑4602433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑