Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	858,557	C→T	E169K (GAA→AAA)	ybiU ←	DUF1479 domain‑containing protein YbiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	858,557	0	C	T	100.0%	23.6 / NA	10	E169K (GAA→AAA)	ybiU	DUF1479 domain‑containing protein YbiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

ATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGACGTGAAGCGCTGAGCGTCCCGAAGAAATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCC  >  NC_000913/858345‑858732                                                                                                                                                                                                                     |                                                                                                                                                                                aTGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACGTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTcn                                                                                                                                           <  1:24900/251‑2 (MQ=255)                                 gATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCAGCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTgc                                                                                                           <  1:216185/251‑1 (MQ=255)                                          tAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCAAGTGCCCCGGAGACGGTACGCGCTCCAAGACCTTCGGACGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGCAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGAGCATTc                                                                                                                                            >  1:233557/1‑209 (MQ=255)                                           aaaGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGAcc                                                                                                 <  1:9216/251‑1 (MQ=255)                                                              cgcTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGCCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGAtt                                                                                                                                                               >  1:212050/1‑170 (MQ=255)                                                                                                                     cAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATgg                                                                                        <  1:84510/186‑1 (MQ=255)                                                                                                                          ccTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCAGTTCTTCACGCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGACGTGAAGCGCTGAGCGTCCCGAAGAAATTATCGCCGGGGCCTTTGTAGACCTCGTc                  <  1:130098/251‑1 (MQ=255)                                                                                                                                           gggCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGACGTGAAGCGCTGAGCGTCCCGAAGAAATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTcc  >  1:221260/1‑250 (MQ=255)                                                                                                                                                naCGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTACATTACTTTTAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCAATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGCCCAGTAGATGGGGTAAATCTCGGGAc                                                                         >  1:25820/2‑174 (MQ=255)                                                                                                                                                                           tGACGCTCACATCCGGGTTAATCCATGGCTTTCCATCGCTTGTAAATGTCCACAGACGATTTAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGcn                                                                                                                        <  1:48583/100‑2 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                ATGTGCCGCATGCCAGGGATCATATTGCGCCAGATTGCCATTAAAGACGTTGGCGAAAACGCGCTGATACGCTGGAAGCAGCCAGCGTTCCAGTGCCCCGGAGTCGGTATGCGCTCCAAGACCTTTGGAGGTCGTTCCGGGCGGACGGCGGCGGATACGGTCAGGGTAGATGACGCTCACATCCGGGTTAAACCATTGCTTTCCATCACTTTCAAATGTCCACAGACGATTGAGAAACGACTGCGCATTCGCCATTTCTTCACTCTGGCGGGCCTGCATTTGCGCCTGCGACCAGTAGATGGGGTAAATCTCGGGACGTGAAGCGCTGAGCGTCCCGAAGAAATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCC  >  NC_000913/858345‑858732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑