Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,052	G→A	P512S (CCG→TCG)	yoaE ←	TerC family inner membrane protein YoaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,052	0	G	A	100.0%	15.4 / NA	9	P512S (CCG→TCG)	yoaE	TerC family inner membrane protein YoaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

CGGAGGGCGTGGAAACGCAAGAACAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGT  >  NC_000913/1899844‑1900204                                                                                                                                                                                                                 |                                                                                                                                                         cGGAGGGCGTGGAAACGCAAGAACAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTTTGCAAGGGTATTTGTAAGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGATTGCTCTTTAACAATGCGAAccc                                                                                                                                   >  1:36513/1‑231 (MQ=255)        ngTGGAAACGCAAGAACAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTAATCTTCATCGTGCGCCGATTGCTCTTTAACACTGCGAACCAGATCAACACGATAATCATTGGCTTc                                                                                                          >  1:48136/2‑250 (MQ=255)                    aGAACAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTATGCAAGGGTCTTTGTACGCAAAGACGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGTCAACCGCCGCCCCAACGGCATAATGGGAATATCACGCACCTTATGCCGTTACGTTAACGCTTCATCATCTTCATCTTGCGTCGATTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGTTTAAACGTTGGTGat                                                                                              >  1:164812/1‑250 (MQ=255)                                             cATGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTGCATCGTGCGCCGATTGCTCTTTaan                                                                                                                                              <  1:77846/177‑2 (MQ=255)                                                                                 caGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATACTGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGATTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGt                                                                                                 >  1:180703/1‑186 (MQ=255)                                                                                               aCGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGATTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGGGACCATTTGCCGAGATCACGAGGCCCGCGAcn                  <  1:64056/251‑2 (MQ=255)                                                                                                                                            ccgGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGATTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACatc                                                                            <  1:84024/148‑1 (MQ=255)                                                                                                                                                  tAATGGACATATCACGCCCATTATGCCGTTACGTTAAAGCTTACTCATCTTCATCGTGCGCCGATTGCTATTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCACTGATGGTGATATGc                                                                                          >  1:129163/1‑128 (MQ=255)                                                                                                                                                                                    taACGCTTACTCATCTTCATCGTGCGCCGATTGCTCTTTATCACTGCGAACCAGATCAGCAAGATAATCATTGGCTTAAATGATGGTGATATGCAGAGGCACTACCTCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATAtcatcgt  >  1:237241/1‑182 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                         CGGAGGGCGTGGAAACGCAAGAACAGGCGAAGTATTTGCGCCGTCATGGCGTGCATATTTTGCAAGGGTATTTGTACGCACAGCCGATGCCGCTACGTGATTTTCCCAAATGGCTGGCGGGCAGCCAACCGCCGCCCGCCCGGCATAATGGACATATCACGCCCATTATGCCGTTACGTTAACGCTTACTCATCTTCATCGTGCGCCGGTTGCTCTTTAACAATGCGAACCAGATCAACACGATAATCATTGGCTTCAATGATGGTGATATGCAGTGGCCCTACATCAATCACATCGCCCACACGGGGAATGTGACCATTTGCCGAGATCACGAGGCCCGCGACCGTCGCGATATCATCGT  >  NC_000913/1899844‑1900204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑