Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,867,262	C→T	L119F (CTT→TTT)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,867,262	0	C	T	85.7%	13.4 / NA	7	L119F (CTT→TTT)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (3/3);  minor base G (0/1);  total (3/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACGGCATGGAAGACGCGATTGAACAGATTGTCTCTTATCTGAAACACGCGGCTCAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTG‑GGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCA  >  NC_000913/1867139‑1867383                                                                                                                             |                                                                                                                          ttttACGGCATGGATGACGCGATTGAACAGATTGGCTCTTATCTGAATCTCGCGGCTTAGGGGTTTTAAAAGAAGAAACAATTCCTGTATCTGCGGGGGCCGGTG‑TGTGGGGGTTAATCATCTTTCGCTGAGCGATTGAAATCATTAATTCAGCTCGTTCCGAGTGATGTTTTTAGCGCTATTGGTTAGCTTAGTCCGGTCAACGATCATCCGTTCTATCTTTTCAATCCGCAGGAAGATGCGCa  <  1:184499/245‑1 (MQ=255)                    ngATTGAACAGATTGTCTCTTATCTGAAACACGCGGCTCAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTG‑GGTGGGGGTAAATCATCGTTTg                                                                                                                        >  1:203758/2‑108 (MQ=255)                         naaCAGATTGTCTCTTATCTGAAACACGCGGCTCAGGGGCTGGAAGAGAAGAAANAAATCCTGTNNCTNCGGGGNCNNNTN‑NNTGNGGGTAAATCATCGTTTGCTNAGNNACTGAANTCATTAATGCAGCTCGTACCGATTTa                                                                                <  2:56817/142‑1 (MQ=255)                         gAACAGATTGTCTCTTATCTGAAACACGCGGCTCAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTG‑GGTGGGGGTAAATCATCGTTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTa                                                                                >  1:56817/1‑143 (MQ=255)                                                     cgGCTCAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCGGCTGGGGCCT‑TGTGGTGGGGGTAAATCATCGGTTGCGGAACGACTGAAATCATTAATGCATCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTa                                                      <  1:238050/141‑1 (MQ=255)                                                                        gaagaaACAAATCCTGTATCTGCTGGGGCCTGGG‑GGTGGGGGTAAATCATCGTTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAgc                                                                      <  1:126373/106‑1 (MQ=255)                                                                                                           ggtgggGGTAAATCATCGTTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATCGAGCGCGAACGGCGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATcc                 >  1:171941/1‑125 (MQ=255)                                                                                                                             |                                                                                                                          TTTTACGGCATGGAAGACGCGATTGAACAGATTGTCTCTTATCTGAAACACGCGGCTCAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTG‑GGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCA  >  NC_000913/1867139‑1867383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑