Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,843	C→T	C245C (TGC→TGT)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,843	0	C	T	100.0%	23.5 / NA	11	C245C (TGC→TGT)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

TGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCA  >  NC_000913/4217605‑4218014                                                                                                                                                                                                                                               |                                                                                                                                                                            tGAATGCCTTTGAACTGATGAAAGCGATGAATGAAGCCGGTGCAGCGGCAGTTAACTTCGAAGATCCACAGGCGACAACGAAGAAAACCGGCCACATCGGCGGCAAAGTTTTAGTGCCACCTCAGGAAGCTATTCCGAAACCGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGACCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTaaga                                                                                                                                                                  >  1:105519/1‑247 (MQ=255)                                                 cAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACt                                                                                                                 <  1:116442/251‑1 (MQ=255)                                                           cGAAGACCAGCTGGCGTCAGTTAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTTCCAACTCAGGAAGCTAGTCAGAAAATGGTCGCGGCGCGTCTGGCAGCGGACGTGACGGGAGTTCCAACCCTGCTGGTTGCCCGTACCGATGATGATGCGGCGGAGCTGATCACCTCCGATTGTGATCCGTAGGACAGCGAATTTAGTACCGGCGAGCGTACCAGTGAAGGCTGCTTCCGTCCTCATGCGGGc                                                                                                        <  1:37799/250‑1 (MQ=255)                                                                                                      ggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATgn                                                            <  1:155640/251‑2 (MQ=255)                                                                                                      ggcAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGc                                                            <  1:14221/251‑1 (MQ=255)                                                                                                                              naaGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGAAGTGACGGGCGTTCCCACCCTGCTCGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCAACTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCgcgc                                                                                                                                           >  1:28289/2‑145 (MQ=255)                                                                                                                                    aTTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGAATGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTAc                                                                                                                                       >  1:13687/1‑146 (MQ=255)                                                                                                                                                   gcggcgCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCGGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGgc               <  1:56554/251‑1 (MQ=255)                                                                                                                                                                cAGCTGACGTGACGGGCGGTCCAACCCTGCTGGTTGCCCGTACCGATGCGGATGCGGCGGATCTGATCACCTGCGATTGTGACCCGTATGACACGGAATTTAGTACTGTCGAGCGTACCAGTGAGGGCTGTTTCCATACTTATGCGGGCATTGATCAAGCGATCAGCCGGGGTCTGTTGTAGGCGCTATATGCTGATGTGGTCTGGTGTGTAGCCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGca  <  1:122491/251‑1 (MQ=255)                                                                                                                                                                                 gTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTTACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCa                                                                                                >  1:13319/1‑140 (MQ=255)                                                                                                                                                                                   tNNAACCCTNNTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGTGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTAc                                                                                                                                       <  2:13687/99‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                            TGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGGGCGTTCCAACCCTGCTGGTTGCCCGTACCGATGCTGATGCGGCGGATCTGATCACCTCCGATTGCGACCCGTATGACAGCGAATTTATTACCGGCGAGCGTACCAGTGAAGGCTTCTTCCGTACTCATGCGGGCATTGAGCAAGCGATCAGCCGTGGCCTGGCGTATGCGCCATATGCTGACCTGGTCTGGTGTGAAACCTCCACGCCGGATCTGGAACTGGCGCGTCGCTTTGCA  >  NC_000913/4217605‑4218014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑