Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,253,284	C→T	A90V (GCG→GTG)	ubiA →	4‑hydroxybenzoate octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,253,284	0	C	T	100.0%	28.9 / NA	12	A90V (GCG→GTG)	ubiA	4‑hydroxybenzoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGT  >  NC_000913/4253087‑4253472                                                                                                                                                                                                      |                                                                                                                                                                                             cgcgTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAGCCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGt                                                                                                                                         <  1:234169/251‑1 (MQ=255)                                        ggtggCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGATGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGc                                                                                                                                                        >  1:222963/1‑197 (MQ=255)                                             cGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTNg                                                                                             <  1:199050/251‑1 (MQ=255)                                                                                                       gNTGCGCGCTGACGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAc                                   >  1:199727/1‑251 (MQ=255)                                                                                                           cgctcTGCCGGATGTGTGGTGAATGATTATGCTGTCCGCAAGTTTGATGGTCATGTTAAGCTCGCCGCGATCCGTCCTCGTCCTAACTGCGTGATAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTatt                                                                                                                                  <  1:241275/148‑3 (MQ=255)                                                                                                                 gCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt                         >  1:187281/1‑251 (MQ=255)                                                                                                                                         gCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGt  <  1:220741/250‑1 (MQ=255)                                                                                                                                                          tGGTCNTGTTCNGNNCACGGCGAACCGACCACTTNCCAGCGGCGTGGTAANAGAGNAAGAGncgng                                                                                                                                                                         >  2:106456/1‑66 (MQ=255)                                                                                                                                                          tGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCGAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGc                       <  1:106456/212‑1 (MQ=255)                                                                                                                                                           ggTCATGTTAAGCGCACGGCGAACCGACCANTTCNCAGCNGCNTTGTAACAGAGAAAGNGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACNNGNNNTNACGCTGaa                                                                                                                   <  2:173344/119‑1 (MQ=255)                                                                                                                                                           ggTCATGATAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGTGGTACCAGCGACAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGCTTTCGCTTTTATTGGTGCTGaagatgac                                                                                                                   >  1:173344/1‑112 (MQ=255)                                                                                                                                                                               gAACCGACCACTTCCCAGCGGCGTGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTATTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTgn       <  1:65257/207‑2 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                             CGCGTTACTGCTGCTCTGGCCAACATTATGGGCGTTGTGGGTGGCGACACCGGGCGTTCCCCAGCTCTGGATCCTGGCGGTGTTTGTCGCGGGTGTCTGGCTGATGCGCGCTGCCGGATGTGTGGTGAATGATTATGCTGACCGCAAGTTTGATGGTCATGTTAAGCGCACGGCGAACCGACCACTTCCCAGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGT  >  NC_000913/4253087‑4253472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑