Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,158	G→A	D84N (GAC→AAC)	yeaO →	DUF488 domain‑containing protein YeaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,158	0	G	A	100.0%	24.5 / NA	11	D84N (GAC→AAC)	yeaO	DUF488 domain‑containing protein YeaO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/5);  total (6/5)

GAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCA  >  NC_000913/1877035‑1877352                                                                                                                            |                                                                                                                                                                                                   gAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGACGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCANAAANNNNncc                                                                                                                                                                                >  2:160081/1‑143 (MQ=255) gAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGACGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTggn                                                                                                                        <  1:160081/200‑2 (MQ=255)    aTCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGGCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAAcc                                                                                                                                        <  1:124440/181‑1 (MQ=255)                                   ttCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCACCTCTACTCAGCAAANAACNNNNNGcnnnnnnnnnn                                                                                                                                    >  2:152859/1‑144 (MQ=255)                                             aGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGTAGCACTGGCGCAACAAAAGCCAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAACAACAAACCCCACGCaacaccc                                                                                                                                       >  1:35728/1‑135 (MQ=255)                                                    gATTATGCAACCTTTCGCGAGCAATATCCTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCTCTGACCCCGCTCTACTCAGCAAAAAACCCCACACACAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTGGTGATTTTAGt                                                                                           <  1:144472/178‑1 (MQ=255)                                                                     ngAGCAATATCTTGCAGAACTGGCGCAAAACGAGCAAGAAGGAAAGCGCCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGAGCTCGCCGACTGGCTAGGTAGCTTGTGGTTTTAGTACTGCCTCAGGCGGTTATTGTTTACCAGCCGGATGGTAACGCAGCCACAAGACCCATTCATCAATAGTTTCACCGTCGGGTAATTTGcc  >  1:150813/2‑249 (MQ=255)                                                                                   nnnnnnngnccnnacacNAGCAAGAAGGAAAGCGGCTGGCGANCATCGCCAAAAAACAGCCGCTGACCCTTCTCNANTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGNTACGTAGCTTNTGANNTTAGNANANCATCCGGCGGTTATTTTTCACCAGCCGGANGGTCACGCCGCCACAATGCCCATNNATNNNTCNNTTCAc                  <  2:190267/207‑1 (MQ=255)                                                                                          ggCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTAACGCCGCCACAATGCCCATTCATCAATCGTTTccc                  >  1:190267/1‑211 (MQ=255)                                                                                           gcgcAACACGAGCAAGAAGGAAAGCGGCTGGCGAACATCGCCAAAAAAAAGCCGCTGACCCTGCTCTACTGAGCAAAAAaca                                                                                                                                                    >  1:238011/1‑82 (MQ=255)                                                                                                                     gCTGGCGAACAGCGCCAAAAAACAGCCGCTGACCCGGCTCTACTCAGCAAAAAACTCCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCGTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCAc                                                                  <  1:185550/138‑1 (MQ=255)                                                                                                                            |                                                                                                                                                                                                   GAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCACGGCGAAGTCGTCGATTATGCAACCTTTCGCGAGCAATATCTTGCAGAACTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCTACTCAGCAAAAAACACCACGCAGAACCATGCGCTGGTGCTGGCCGACTGGCTACGTAGCTTGTGATTTTAGTACAGCATCCGGCGGTTATTTTTCACCAGCCGGATGGTCACGCCGCCACAATGCCCATTCATCAATCGTTTCACCGCCCGGTAATTTGCA  >  NC_000913/1877035‑1877352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑