Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,915	C→T	M12I (ATG→ATA)	ybiA ←	N‑glycosidase YbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,915	0	C	T	100.0%	26.5 / NA	12	M12I (ATG→ATA)	ybiA	N‑glycosidase YbiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCG  >  NC_000913/832695‑833156                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  gCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGTAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTATAAATTTATGATTGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACg                                                                                                                                                                                                                     <  1:163238/251‑1 (MQ=255)                                              gggAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAACTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGc                                                                                                                                                                                                        >  1:34433/1‑219 (MQ=255)                                                              gATCTCTACGCGGTATTTTTCATCACGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGACTTTCCCGTAAACCTTGCTGGGCGAGGCGGCAAAGATAGAAAAATCACCGTCGTCATCGCTGGTGCTGTCGAAATTTCTGTCGGTGTCTTGTATGACGTGTTGGATCCTTTGTGc                                                                                                                                                                                                                            >  1:249877/1‑183 (MQ=255)                                                                                                      tGAAAATAGTGTTCTGAGGTAGGCCAGGTTTGCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCAGCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGGCGTGTTGGATCCTTTGTGc                                                                                                                                                                                                                            <  1:222071/143‑1 (MQ=255)                                                                                                                    tGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAg                                                                                                                                                                                 <  1:21516/172‑1 (MQ=255)                                                                                                                              cAGGCTTTCCCGTCAACCTTGATGGGCCAGGCGGCCAAGTTAGAAAAATGCCAGTAGTCCTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTCAATCGCATTATGTTGGTGGTTATTGCGCGCCGCTTTCCAGAAACAGACAAACCATTACCACTGAAAACCGAAAAATGCAACAATCTTGGATGTTTATAAAGTATTTCAGGTTTtctc                                                                                         >  1:167415/1‑250 (MQ=255)                                                                                                                                                       nccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGCGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATACTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAgc                                                               >  1:48248/2‑251 (MQ=255)                                                                                                                                                           agcggcAAAGATAGAAAAATCAACGTAGTAAGCGCTGGTGATGTAGAAATATATGAAGGTGTCTTGTATGACGAGTTGGAGCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGAGGATATTGCGAGACGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGATGTTTATACAGTATTTCAGGTTTTCTCATGGTATTAACCGCAGCGCTTAAAGCGCa                                                            >  1:240045/2‑250 (MQ=255)                                                                                                                                                                aaaaGTTAGAAAAATCACCGGAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTgn                                                      <  1:204032/250‑2 (MQ=255)                                                                                                                                                                                aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCGCAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGgcg                                      <  1:44330/251‑1 (MQ=255)                                                                                                                                                                                                                    ggTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCCTTATGTTGGTGGTTATTGCGAGCAGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACCGATCCCCGACTTTATTCCCCGTgcg  >  1:245564/1‑251 (MQ=255)                                                                                                                                                                                                                    ggTGTCTTGTATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAATCGCATTATGTGGGTGGTTATGGCGAGCCGCTTTCCAGAAACAGAAAAACCATTCCCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTgc   <  1:60383/250‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  GCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCG  >  NC_000913/832695‑833156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑