Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	129,296	G→A	S462N (AGC→AAC)	lpd →	lipoamide dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	129,296	0	G	A	100.0%	14.1 / NA	8	S462N (AGC→AAC)	lpd	lipoamide dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

CTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCAAGCTGATTTTCGACAAAGAATCTCACCGTGTGATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTC  >  NC_000913/129062‑129535                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                cTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCCAGCTGATTTTCGACAAAGAATCTCACCGCGTGATCGGTGCTGCGATTGTCGGTACTAACGGCGGCCAGCCGGCGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGGTGCTGAAGACATCGCAATGACCATACACGCGCACCCGACTCTGACCGAGTCTCTGGGCATGGCGGCAGAAGTGGTCGAAGGACACATTACCGACCTGCCg                                                                                                                                                                                                                                 >  1:189968/1‑251 (MQ=255)                                                                                                                    gggTGAAATCGGCCTGGCAATCGAAATGGGTTTTGATGCTGAAGACATCGCACTGACCTTCCACGCGCGCCCGACTCGTCACGAGTCTGGGGGCCTGGCGGCAGAAGTGTTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAc                                                                                                                                                          <  1:230132/207‑1 (MQ=255)                                                                                                                          aaTCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACg                                                                                                        >  1:13972/1‑251 (MQ=255)                                                                                                                                                                 cATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGCAGTGTTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTc                                                                                                            >  1:110642/1‑208 (MQ=255)                                                                                                                                                                          gACCATCCACGCGCCCCCGACTCTGCACGAGTCTGTGGACCTGGCGGCAGAAGTGTTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCTTTATTCAGct                                                         <  1:73200/250‑2 (MQ=255)                                                                                                                                                                                                            tgtggCCTGGCGGCAGAAGTGTTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAATGAATGGTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCTCCTAGCCACGCCGCTTTTTCTACGTCTGCAATGTGCCTTTCCAGTCGGCTTGCTCCACGTTCAGAGAGACGTTCGCATActgctg                                                                              <  1:229026/192‑1 (MQ=255)                                                                                                                                                                                                                                gtTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGGGAAGAAGAAGACATTTTTCGTTTGCCGGCACATCAGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTa                                           >  1:103290/1‑210 (MQ=255)                                                                                                                                                                                                                                gtTCGAAGGTAACATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTtctc  >  1:11334/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                CTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCAAGCTGATTTTCGACAAAGAATCTCACCGTGTGATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTC  >  NC_000913/129062‑129535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑