Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,855	G→A	L298F (CTC→TTC)	kgtP ←	alpha‑ketoglutarate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,855	0	G	A	100.0%	35.9 / NA	14	L298F (CTC→TTC)	kgtP	alpha‑ketoglutarate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

CCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCC  >  NC_000913/2724663‑2725050                                                                                                                                                                                                 |                                                                                                                                                                                                    ccTTCAGTATTCCACTGATTAATGTATAAAAACTCACTATCAGCAGGGCACTCATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGAAACGGTAAAAACGGCTGCGAGCGAACCGAAACATAGCATTGAATTACGGCGATCAATCTTATCCGACTGCGGGCCAATGAATCGGTGAATAAGCATAAATTCAACCAATGCGGCAGTCATAATGCCACTCGCCACGTTg                                                                                                                                           <  1:43810/251‑1 (MQ=255)                                                                            aaGGCGAGGAAACGTTTTGCAATGCGGAGAGAATAGAAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGTTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCgg                                                                                                                                                                     <  1:243235/150‑1 (MQ=255)                                                                                                        gagaATAGAAACGGTAAAAATGGCTGCCAGCGAAACGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAAACatga                                          >  1:201525/1‑245 (MQ=255)                                                                                                          gaATAGAAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCNCTCNNNNNGtnn                                                                                                                                           >  2:158725/1‑144 (MQ=255)                                                                                                          gaATAGAAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCgn                                                                                                                             <  1:158725/160‑2 (MQ=255)                                                                                                                       aaaaaGGGCTGCCAGCGAACCGAAACATAAAATTGAGGTACGGAGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGTAGTCATAAGGCCACGCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGGAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCTGTAAAACGGAGAACCATGATGAATGCACGGCGATTgcgc                      <  1:103329/250‑1 (MQ=255)                                                                                                                        aaaaTGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAAGACAAACAATGCGGCAGTCATAATGCCACTCg                                                                                                                                                   <  1:73461/124‑1 (MQ=255)                                                                                                                                     gtgAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACATCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTaaaa                                                       >  1:62485/3‑203 (MQ=255)                                                                                                                                        aaCCGACACATAACATTGAGGTACGGCGACCAATCTTATACGACAGCGAGACAATGAATGGTTGAAAAAGCATGAATAAAAACAATGCGGCAGTCATAATGCCAATCGCCACGTTGGCATGCATTCCCGCAGTATTTACAAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCATTTCaga       >  1:176572/1‑248 (MQ=255)                                                                                                                                            gAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATcc  >  1:21961/1‑249 (MQ=255)                                                                                                                                               ncanaacntNNANNTACNGCGACCAATCTTATCCGACAGNNCGCCAATGANTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCANGTTGGCATGNNTTNCCGCANTNNNTNNNAGNTACATCTGCATATAAGTAGNGANNGTATAGNAACAAAGGGAGCCCGCAGCGGTaaaa                                                       <  2:62485/192‑1 (MQ=255)                                                                                                                                                                    aCCAATCTTATCCGACAGCGCGCCAATGAATGGTTGAATAAGCAT‑AATACAAACAAGGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATGTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCt             <  1:128793/213‑1 (MQ=255)                                                                                                                                                                           ttATCCGACAGCGCGCCAATGAATGGTTGACTAAGCATGAATACAAACAATGCGGCAGTCATAATGACACTCGCAACGTTGGCATGCATTCCCGCAGTATTTACCAg                                                                                                                 >  1:110901/1‑107 (MQ=255)                                                                                                                                                                               ccGACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGAAGTCATACTGCCACTCGCCACGTTGgca                                                                                                                                        >  1:135899/1‑80 (MQ=255)                                                                                                                                                                                 gACAGCGCGCCAATGAATGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCAAGTTGGCATGCACTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGCATAGAAAAAAAGGGAGCCCGCAGCGGTaaa                                                        >  1:35713/1‑158 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                    CCTTCAGTATTCCACTGATTGATGTATAAAAACTCACTATCAGCAGGGCACACATCACCAGACCAAAAGCGGCATAAGGCGAGGAAACGTTTTGCAATGCTGAGAGAATAGGAACGGTAAAAATGGCTGCCAGCGAACCGAAACATAACATTGAGGTACGGCGACCAATCTTATCCGACAGCGCGCCAATGAGTGGTTGAATAAGCATGAATACAAACAATGCGGCAGTCATAATGCCACTCGCCACGTTGGCATGCATTCCCGCAGTATTTACCAGATACTTCTGCATATAAGTAGTGAAGGTATAGAAACAAAGGGAGCCCGCAGCGGTAAAACCGAGAACCATGATGAATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCC  >  NC_000913/2724663‑2725050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑