Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,878,257	G→A	G181G (GGG→GGA)	dgcP →	diguanylate cyclase DgcP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,257	0	G	A	100.0%	29.3 / NA	11	G181G (GGG→GGA)	dgcP	diguanylate cyclase DgcP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

GATCATTCTATGGCACGCTTTGCGCCGCCAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAA  >  NC_000913/1878070‑1878446                                                                                                                                                                                            |                                                                                                                                                                                               gatCATTCTATGGCACGCTTTGCGCCGCCAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAAC‑AGACTACCGAATCGGCGGGCGATTTTT‑GAAAATc                                                                                                                                                                >  1:219313/1‑218 (MQ=255)                        gccgccAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCCGCATCTTAACCATAAGATAATGATCGCGtt                                                                                                         >  1:191774/1‑251 (MQ=255)                                                                                                     atatatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTg                            <  1:39810/251‑1 (MQ=255)                                                                                                         atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGAGCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCg                         <  1:41718/250‑1 (MQ=255)                                                                                                               aaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCt                  <  1:5662/251‑1 (MQ=255)                                                                                                                    gtcACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGGGGCGATCTGTTTCTCATTCAGGTTGGCGCGCGCCTTAAt              >  1:89023/3‑250 (MQ=255)                                                                                                                                nnaGNNNNNCGANGCCAATGCCGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTCACCATAAGATAATGATCGCGt                                                                                                          <  2:157185/144‑1 (MQ=255)                                                                                                                                 naGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGAATACCGAATCGGCGGGCGATTTTTTGAAAATATGACGCCACTGTTTTCCCTCCCCCGGCATCTTAACCATACGGTAATGTTCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGGGCCTTAATACCCTCCAGCaa  >  1:28351/2‑250 (MQ=255)                                                                                                                                              ccAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGcccn                                                                                                                                       <  1:67576/103‑2 (MQ=255)                                                                                                                                                aaTGCTGCGCTGATTTCGCAATCGTATACCGACTCGTTAACCGGACTACCGAATCGGCGGGCGATTTTT‑g                                                                                                                                                                      <  1:231127/70‑1 (MQ=255)                                                                                                                                                                                      aaCCGGACTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTTCCTCCCCCGGCATCTTAACCCTAAGATAATGATCGCGTTTATCGCTCTGGATAACTTCAAATTAATc                                                                             >  1:219960/1‑121 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                               GATCATTCTATGGCACGCTTTGCGCCGCCAGCAGTGAGAAGCGCCAGTGGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTT‑GAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAA  >  NC_000913/1878070‑1878446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑