Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	473,447	C→T	S161F (TCT→TTT)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	473,447	0	C	T	92.9%	34.0 / NA	15	S161F (TCT→TTT)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (5/8);  minor base G (0/1);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTCTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGC  >  NC_000913/473255‑473606                                                                                                                                                                                                 |                                                                                                                                                                tcGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTGTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTg                                                                                                         <  1:97829/249‑1 (MQ=255)                  ttGATGCTGAAAAACATCGAACTGACTGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGTGGCGGTGGTTTGCTGGCTtc                                                                                                                 <  1:210294/224‑1 (MQ=255)                          gAAAAACATCGAACTGACGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCNc                                                                                                             <  1:199221/220‑1 (MQ=255)                            aaaaCATCGAACGGACGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTGTGCCTGCATTACCGTTGGCTTGATAGTTGGGGAGCTGGCGGATCGAATCCGCTTCTCAGCTGCGTTGATTTTCGGTGTGGTATGGCTGACGCTCTGTTACATTCCGTTTGCGCATATGGTGTGGGGCGGTGCTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCgtggtg                                                                            <  1:183408/251‑1 (MQ=255)                                             gcggTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTCTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAAt                                                           >  1:249183/1‑251 (MQ=255)                                                                                                                 cGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGGGGTATGGCTGACGCTCTTTTAACTTCCGATTGCGCATATGGAGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACCTTCACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTc             >  1:115422/1‑229 (MQ=255)                                                                                                                    cTTGATAGTTGGGGCGCTGGCGTAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTgg                                                                                      <  1:86004/153‑1 (MQ=255)                                                                                                                     ttGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGNGCTNNNNNTcnn                                                                                            >  2:158817/1‑144 (MQ=255)                                                                                                                     ttGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTcgn                                                                                            <  1:158817/146‑2 (MQ=255)                                                                                                                       gATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCACCTGTGTTGATTTTCGTGGTGGCATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTg                                                                                                                      >  1:244814/1‑118 (MQ=255)                                                                                                                                      ggCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTgg                                                                                                                     <  1:2930/104‑1 (MQ=255)                                                                                                                                             cGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGc  >  1:131838/1‑212 (MQ=255)                                                                                                                                                aTCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTTACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACTGTGCGCTGGATTTCGCGTGTGGCACCgtgg                                                                              <  1:213497/133‑1 (MQ=255)                                                                                                                                                                                       cTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCANNGTGGTGnnc                                                                         >  2:7289/1‑99 (MQ=255)                                                                                                                                                                                       cTGACGCTCTTTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGgcg                                          <  1:7289/130‑1 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                TCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTCTCTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCGCTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGCGCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGC  >  NC_000913/473255‑473606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑