Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,243,528	C→T	intergenic (‑12/+3)	malG ← / ← malF	maltose ABC transporter membrane subunit MalG/maltose ABC transporter membrane subunit MalF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,243,528	0	C	T	100.0%	37.6 / NA	14	intergenic (‑12/+3)	malG/malF	maltose ABC transporter membrane subunit MalG/maltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

GCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTCCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGA  >  NC_000913/4243298‑4243754                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   gCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCTGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAAGCAAACTTCATTcg                                                                                                                                                                                                                <  1:166006/251‑1 (MQ=255)  cGACCATCAGCCTGTTCAACGCTAAAACCTAACGCAAGTTTCCAGTGATCCC‑GGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTcgcg                                                                                                                                                                                                              <  1:13394/251‑1 (MQ=255)            ccTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGCTATCCCAGGAGATTTGCTCCCCGATAACCCTGCCGGTCGCAAAGTTTCCCTAACGCAGCGAGAAAGCGACCACAATCAGCAGCGGGACCATAATCGCTGCGGTCTGATGTACCCGTAGAAGCAGAGTAACAAATAAACGAGCTTTTTGCGATTTCGGTTGGCCCATTGCCATTTTTGTTATCTTCATTACTCATACTTCATTcaggagtggatg                                                                                                                                                                                                      >  1:140435/1‑239 (MQ=255)               gTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGACTTCGGTTGGACCATTTCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAgn                                                                                                                                                                                                   <  1:51220/250‑2 (MQ=255)                                                                cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAAc                                                                                                                                                                                                                        >  1:70527/1‑180 (MQ=255)                                                                               gCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCTCCGCCCATCAGCAGCGGGCAAATAATTGATAAGATACAGAGTAGTAGTAGAAGGTGCGTAATAAATAACCGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGAGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGa                                                                                                                                   >  1:162884/1‑250 (MQ=255)                                                                                    tCGCAAAGTTTCCCTGAAGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGAAGTCCt                                                                                                                             >  1:62810/1‑251 (MQ=255)                                                                                      gCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATtgc                                                                                                                                                 >  1:141359/1‑229 (MQ=255)                                                                                                                       acCATCAGCAGCGGGAACATAATCGCTGCGCTAAAAAGAAGCAGTAGCAGATGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACAAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCCGACCGAAGTCCTGACCCCCGCCGCCTTCAAACGCGATGCGGTAGgtg                                                                                          >  1:216886/1‑251 (MQ=255)                                                                                                                                           aaTCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGAAGTCCTGACCCCCGCCGCCGTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCgg                                                                       <  1:212411/250‑1 (MQ=255)                                                                                                                                                                                 taaataaaCGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGc                                                                                                                                                       <  1:10509/132‑1 (MQ=255)                                                                                                                                                                                            gCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATTTTCCTTAATTAAACTTCATTCACGTGGCTTTCAGGTTCACTATCGCCAGCGcacccact                                                                                                                                                                           >  1:244154/1‑100 (MQ=255)                                                                                                                                                                                                                gACCATTGCCATTTTTGTTATCTTCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGa  >  1:56288/1‑250 (MQ=255)                                                                                                                                                                                                                               tGTTATCTTCCTTAATCAAACTTCATGCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATtg                                                                                                                                                  <  1:129933/91‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   GCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCAGGTGAGTAATAAATAAACGAGCTTTTTGCGATTTCGGTTGGACCATTGCCATTTTTGTTATCTCCCTTAATCAAACTTCATTCGCGTGGCTTTCAGGTTCACTATCGCCAGCGCACCCACCAGCAGGAAGATCAGCGTGGCAATTGCTGCCGCCAGACCGAAGTCCTGACCCCCGCCGCCTTCAAAAGCGATGCGGTAGGTGTAGTTAACAAGCAGGTCGGTATAACCGGCTGGCGTGGTCGTGCCAAGACGATCCGGGCCGCCGTTGGTTAACAGTTGAATCAGCACGA  >  NC_000913/4243298‑4243754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑