Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,860	C→T	A718T (GCG→ACG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,860	0	C	T	100.0%	30.0 / NA	13	A718T (GCG→ACG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

CGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCC  >  NC_000913/2819663‑2820086                                                                                                                                                                                                      |                                                                                                                                                                                                                                   gcgCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTTCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTAGTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGTGGGCGATAGCACCTtctn                                                                                                                                                                               <  1:77585/249‑2 (MQ=255)   ncTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCg                                                                                                                                                                             >  1:55466/2‑251 (MQ=255)       aaCACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGccc                                                                                                                                                                                     <  1:1638/239‑3 (MQ=255)       aaCACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGtt                                                                                                                                                                          >  1:110125/1‑250 (MQ=255)                                                            tACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTAC‑‑CTCCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCgg                                                                                                                    <  1:35821/249‑1 (MQ=255)                                                                   aaGATTTGCGCACTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGGGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGACTGCAGCAGTACCCGATTCAGAGATGATGCGTAACAGAc                                                                                                             <  1:193623/251‑1 (MQ=255)                                                                               ctcCTGTGCGGCAGCTTGTTCTGTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCAcc                                                                                                   <  1:88736/249‑1 (MQ=255)                                                                                 ccTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACGGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCGCCGTTTACCGCGTCGATACGACTAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTg                                                                                               <  1:223034/251‑1 (MQ=255)                                                                                                                  aCTCTTTTTCCAGCTGACGCGTACGTTCCATTACTGAGCGCACTTTATCAGCCATATTATTGCTATCGCCTTTCAGCAGATTCGTGACTTCGCTTAAGCGATCTCGGGCTGCATGTACGTTGGCGATGGCACCTTCTCCGGTTCCCGCTTCGATACGACGAACGCCTGCAGCAGTAc                                                                                                                                        <  1:187537/177‑1 (MQ=255)                                                                                                                  aCTCTNNTTNNNGCNNACGCGTACGTTCCAGTACTGAGCNCACTTTATCAGCCAGATTATTGCTATCGCNTNTNAGCANNTGCNTGACTTCGCTNAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCCCCTTCTCCGGTTNCNGCTTCGATACGACGAACGCATGCAGCAGTAc                                                                                                                                        >  2:187537/1‑177 (MQ=255)                                                                                                                                                            cttgaTCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGAGCGCTGTCTGCATGAGCGGTGTCGATAGCACCTGCTCCGGTTACCGCTTCGAGACGACGAACGCCGGCAGCAGTACCCGATTCATAGAAGAGGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCcacac                                                                       <  1:168173/196‑1 (MQ=255)                                                                                                                                                                        gATTATTGCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAAc        >  1:4598/1‑251 (MQ=255)                                                                                                                                                                               gCTATCGCCTTTCAGCAGATGCGTGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGAGGCGATAGCAACTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAgcgca  >  1:100708/1‑249 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                   CGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTTACTGGAAAGATTTGCGCTCTCCTGTGCGGCAGCTTGTTCTTTAAGCTGTTGTAACTCTTTTTCCAGCTGACGCGTACGTTCCAGTACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCC  >  NC_000913/2819663‑2820086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑