Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	779,092	G→A	G9E (GGG→GAG)	pal →	peptidoglycan‑associated outer membrane lipoprotein Pal

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	779,092	0	G	A	100.0%	33.9 / NA	13	G9E (GGG→GAG)	pal	peptidoglycan‑associated outer membrane lipoprotein Pal
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

ACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGC  >  NC_000913/778911‑779265                                                                                                                                                                                      |                                                                                                                                                                              aCAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCa                                                                                                                                          >  1:80618/1‑219 (MQ=255)                                       gTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCAc                                                                               <  1:107264/240‑1 (MQ=255)                                         ttcttcAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCATCTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACTCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGc                                                                   <  1:193880/250‑1 (MQ=255)                                                                  cgTCTTCCGGCAACTGATGGACAGGTCACATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTggg                                                                                        >  1:210890/1‑204 (MQ=255)                                                                       tCCGGCAACTGATGGACAGGACAACTTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGACATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGa                                     >  1:206533/1‑250 (MQ=255)                                                                        ccGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCcaaca                                                                                                                             >  1:210469/1‑161 (MQ=255)                                                                               cTGAGGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAATAGCTGATGATTGCGCTGCCTGTTAGGGCAATTGCGGCATTTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAg                                                                                                  <  1:231475/181‑1 (MQ=255)                                                                                    ggACAGGGCAAATTCCCTGCCTGGTCGCCGTATCTGTGATCATAATTAATTGACTAGTAAAGGAATCATTGCAATGCAACTGAACAAAGTGATGACAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACTAGAACGCCAGCAATGACGGCCGCGAAGGCATGCTGGGTGcc                                                                                    >  1:247874/1‑190 (MQ=255)                                                                                         ggTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATACTTAATTGAATAGTAAAGGCATCATTGAAATGCAACTGAAAAAAGTGCTGAAGGAGATGATGATTGCTCTGCCTGTTATg                                                                                                                                                      >  1:36826/1‑119 (MQ=255)                                                                                            cAAATTCCCTGCCTGGTCGCCGTCTCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATgg                                                                       >  1:91653/1‑195 (MQ=255)                                                                                                          ggTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACGGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGTCATGTTCGTCCAACAAGAACGCCAGCAATGACGTCAGCGAAGGCAGGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCCTCCGAAGAGCAGGCTCGTCTGCAAATGCAACATCTgcagc  <  1:211004/250‑1 (MQ=255)                                                                                                                                                               gCAACTGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGAAATTGCGGCATGTTCTTCAAACACGAACGCACGCAATGACGGc                                                                                                         >  1:120944/1‑94 (MQ=255)                                                                                                                                                                    tGAACAAAGTGCTGAAAGAGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGc                                                                   <  1:117018/127‑1 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                                              ACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTGAAATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGC  >  NC_000913/778911‑779265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑