Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,632,560	C→T	D11N (GAC→AAC)	yhiJ ←	DUF4049 domain‑containing protein YhiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,632,560	0	C	T	100.0%	34.0 / NA	13	D11N (GAC→AAC)	yhiJ	DUF4049 domain‑containing protein YhiJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

CCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTG  >  NC_000913/3632425‑3632756                                                                                                                                        |                                                                                                                                                                                                     ccTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATc                                                                                                                      <  1:101325/216‑1 (MQ=255)          gCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTNCCTNNNNNAg                                                                                                                                                                                     >  2:156961/1‑144 (MQ=255)                              gATATATCATCACCTATTCCATTTGCAATATACCCAAACAGTTCGTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACGCCTCATGTTCGATGGGAAAATAAGTATATTTTGCACAAATCATTCAGCCTc                                                       <  1:42352/250‑1 (MQ=255)                                      atcaCCTATACCATTTGCAATATACCCAAACAGATCTTTAGTGAAGGTGGTAACATGCTCTTGCACCATATCATTTGTTGAGATTGTCGTTGTACTGTTGTTAGtgc                                                                                                                                                                                              >  1:142537/1‑107 (MQ=255)                                                             ncccAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCAACAGTTCCACTTTTCATATTTTACCTCATATTATTTACCTCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCAt                          >  1:55602/2‑248 (MQ=255)                                                              cccAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGaaaa                                                                                      >  1:73597/1‑187 (MQ=255)                                                                 aaaCAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCagan                   <  1:156961/251‑2 (MQ=255)                                                                                  ggTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTCACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTg  >  1:167327/2‑251 (MQ=255)                                                                                         aaCATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGTGCCTGACACAGTTCCCATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATCGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGcc                                                         >  1:228191/1‑189 (MQ=255)                                                                                                       ccATATCATTTGTTGCGATTGTCGTTGTACTGTTGTTAGGGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATg                                                                                            <  1:114036/140‑1 (MQ=255)                                                                                                                      cGATTGTCGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTAcagc                                                                                                                                                >  1:117137/1‑70 (MQ=255)                                                                                                                           ntCNTTGTANTGTTNTTAGTGCCTGCCACANTTCCAATTGTCATAGTTTCNNTNATAGTNTTTAc                                                                                                                                                   <  2:111588/64‑1 (MQ=255)                                                                                                                             cGTTGTACTGTTGTTAGTGCCTGCCACAGTTCCAATTTTTATATTTTAACTCCTATTATTTACCGAGAGGAATAc                                                                                                                                       >  1:161584/1‑75 (MQ=255)                                                                                                                                        |                                                                                                                                                                                                     CCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTTGGTAACATGCTCTTGCACCATATCATTTGTTGCGATTGTCGTTGTACTGTCGTTAGTGCCTGCCACAGTTCCAATTTTCATATTTTACCTCATATTATTTACCGCGAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTG  >  NC_000913/3632425‑3632756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑