Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,277	G→A	S85F (TCT→TTT)	rplS ←	50S ribosomal subunit protein L19

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,277	0	G	A	100.0%	41.0 / NA	16	S85F (TCT→TTT)	rplS	50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/7);  total (9/7)

CATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAACCCATACTTTCACTTCCACGGTATCACCCGGACGGAAGGAAGGTACGTCCTGCTTCATCTGCTCTTGTTCAAGTTGCTTAAT  >  NC_000913/2744035‑2744518                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  cATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAACCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCGCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAATGCTg                                                                                                                                                                                                                                           <  1:248785/251‑1 (MQ=255)                                                   cNGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGtt                                                                                                                                                                                         >  1:199147/1‑251 (MQ=255)                                                                                 gCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAGTGCGGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCGGCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGtn                                                                                                                                                           <  1:51730/251‑2 (MQ=255)                                                                                         aTAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAAACGCTCTTTGATACGAGCAGCCTTCCCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTCTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAAc                                                                                                                                                                                                     >  1:253135/1‑201 (MQ=255)                                                                                                         caATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGCTACGAGCAGCCCTAACAGTCCGCTCAAGAAGGTAGAACAGTTTAGCTTTACGCACAGCACCACGACGTTTGACAAACATGCTGTAAACTACCGGCGAGTGAGTCTGGAAGAAACGCTCAACGCCTTCGACGTTGGAAATTTTACGAACAGAGAATACAGAGTGCAGAACGCgg                                                                                                                                                 >  1:175964/1‑237 (MQ=255)                                                                                                                           tAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGGCTTTCCAGTACGCTCACGCAGGTAGTACATTTTAGCGTTACGAACAGCACCGCGACGTTGGACAAAAATGCTGGCAACTTGGGGAGAGTGATTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAAt                                                                                                                                                                   <  1:145805/201‑1 (MQ=255)                                                                                                                            aaCAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGcc                                                                                                                <  1:74416/251‑1 (MQ=255)                                                                                                                                       ncTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGACCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCCCGACGTTTGACAAAACTGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACg                                                                                                                                                                                                    >  1:31248/2‑156 (MQ=255)                                                                                                                                        cTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGTTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAAGGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCGCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAAtn                                                                                                                                                                  <  1:31762/189‑2 (MQ=255)                                                                                                                                              cGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTAc                                                                                                                                                                              >  1:4812/1‑171 (MQ=255)                                                                                                                                                                 ctctTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAAAAATGCTGTCAACTAcc                                                                                                                                                                                                                                  <  1:58003/100‑1 (MQ=255)                                                                                                                                                                                 gcCTTACCAGTACGCTCACGCAGGTAGTCCAGTTTAGCTTTACGAACAGCACCACGCCGTTTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCa                                                                                                                              >  1:94290/1‑184 (MQ=255)                                                                                                                                                                                                           gtaCAGTTTAGCTTTACGAACAGCACCACGACGGTTGACAAAAATGCTGTCAACTACAGGACAGTGAGTCTGGAAGACACGCTCAACCCCTTCGCCGTTGGAAATTTTACGGACAGTGAAt                                                                                                                                                                   >  1:95769/1‑121 (MQ=255)                                                                                                                                                                                                                        ttACGAACAGCACCACGACGTTTGACAAAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAACCCATACTTTCACTTCCACGGTATCACCCGGACGGAAGGAAGGTACGTCCTGCTTCATCTgn                      <  1:156353/249‑2 (MQ=255)                                                                                                                                                                                                                                           ntttGACAAAAATGCTGTCAACTACCGGAGAATGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAACCCATACTTTCACTTCCACGGTATCACCCGGACGGAAGGAAGGTACGTCCTGCTTCATCTGCTCATGTTc              >  1:67404/2‑238 (MQ=255)                                                                                                                                                                                                                                           gTTTGACAAAAATGCTGTCAACAACCGGAGAGTGAGTCTGGAAGACACGATAAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAACCCATACTTTCACTTCCACGGTATCACCCGGACGGAAGGAAGGTACGTCCTGCTTCATCCGCTCTTGTTCAAGTTGCTtaat  >  1:190515/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  CATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAACCCATACTTTCACTTCCACGGTATCACCCGGACGGAAGGAAGGTACGTCCTGCTTCATCTGCTCTTGTTCAAGTTGCTTAAT  >  NC_000913/2744035‑2744518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑