Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,175	C→T	intergenic (‑227/+45)	yagK ← / ← yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,175	0	C	T	100.0%	27.5 / NA	11	intergenic (‑227/+45)	yagK/yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GCCATACAGGCAGGGCTATTCCTGGTCACTGTTGTCGGTATATACTGGTATCAGATTTTAGTACGGACATACTGATCTGATTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGCCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTT  >  NC_000913/292999‑293402                                                                                                                                                                                 |                                                                                                                                                                                                                                    gCCATACAGGCAGGGCTATTCCTGGTCACTGTTGTCGGTATTTACTGGTATCAGATTTTAGTACGGAAATACTGATCTGATTACTTCTCTTACTGGTGAATACAGGGAGTAAGAGTACACAATGCCATTATATCATACCTATCCAACATCTAAATTTGAGACGAAGGTAGACCCAGTCTGTGATGGCTGTTAAAAAg                                                                                                                                                                                                                 >  1:192750/1‑197 (MQ=255)                           cACTGTTGTCGGTATATACTGGTATCAGATTTTAGTACGGACATACTGATCTGATTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGTCCCAGTCTGTGATGGCTGTTAAAAAGt                                                                                                                                                                                                                <  1:237415/172‑1 (MQ=255)                                                                      tACTGATCTGATTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATGGCTGTTaaaaa                                                                                                                                                                                                                  >  1:4361/1‑127 (MQ=255)                                                                       aCTGATCTGATTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAg                                                                                      <  1:89004/250‑1 (MQ=255)                                                                          gatctgatTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATGGCTGTTAAAAAGtt                                                                                                                                                                                                               <  1:134441/126‑1 (MQ=255)                                                                                                  gNNTACAGGNNGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATGGCTGTTaaaaa                                                                                                                                                                                                                  <  2:4361/99‑1 (MQ=255)                                                                                                   aaTACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAATGTAGCCCCAGTCTGTGATGGCTGTTAAAAAGTTTCGCGGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGATTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTc                                                         <  1:252261/251‑1 (MQ=255)                                                                                                      aCAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTccc                                                       <  1:248797/250‑1 (MQ=255)                                                                                                       cAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGTCTGTGATgg                                                                                                                                                                                                                            >  1:100366/1‑84 (MQ=255)                                                                                                                                                                gaCGAAGGTAGCCCCAGTCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTcc                                            <  1:96593/203‑1 (MQ=255)                                                                                                                                                                           ncccAGTCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGAttt  >  1:69733/2‑234 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                                                    GCCATACAGGCAGGGCTATTCCTGGTCACTGTTGTCGGTATATACTGGTATCAGATTTTAGTACGGACATACTGATCTGATTACTACTCTTACTGGTGAATACAGGTAGTAAGAGTACACAATGCCATTATATAATACCTATCCAACATCTAAATTTGAGACGAAGGTAGCCCCAGCCTGTGATGGCTGTTAAAAAGTTACTCTGCAATTAGCTTCTGGAATTAACCGATGATTCCGTTATTCCAGTGCCGCTTCACAGTCGATAAACTGATACTCAATTTCTTTGCAATCTCAGCCTGAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTT  >  NC_000913/292999‑293402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑