Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,136	C→T	intergenic (+57/‑32)	dnaK → / → dnaJ	chaperone protein DnaK/chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,136	0	C	T	100.0%	17.4 / NA	8	intergenic (+57/‑32)	dnaK/dnaJ	chaperone protein DnaK/chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

GCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAA  >  NC_000913/13986‑14289                                                                                                                                                       |                                                                                                                                                          gcagcagACTGCCGGTGCTGCTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGac                                                         >  1:61723/1‑248 (MQ=255)             cGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAAGTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTcc                                                                                     <  1:135724/209‑1 (MQ=255)                ggCTGATGCTTCTGCAAACAACGCGAAAGATGACGATTTTGTCGACGCTGAATTTGAAGAAGTCAAAGAAAAAAAATAATCGCCCTATAAACGGGTAATTATACGGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGATATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTg                                        <  1:163833/250‑1 (MQ=255)                    gATGCTTCCGCAAACACCGCGAAAGATGCCGATGTTGTAGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCACTATAAAAGGGAAATTATACTGACACGGGCGCAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTcc                                                                                     >  1:197513/1‑202 (MQ=255)                                      ncgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCATAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGc                                                                             >  1:49654/2‑192 (MQ=255)                                           agatgactagtttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAAGAATCGCCCTATAAACGGGTAAGTATACTTACACGGGCGAGGGGGAATTTCCGCTCCGCTCGTAGATTCAGCTAGGTGCAATTTAAAAAAGATGGCTAGGCAAGATTATGACGAGATTTTAGTCGGTTCAAAAACAGCCGAAGAGCGTGAAAACAGAAAGGCCTACAAACGCCGGGCCATGAAATACCACCCGGACCGTAAc             <  1:144979/240‑1 (MQ=255)                                              tGACGATGGTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAATCTGGTAATTATACGGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCAt                                                                                                                                                    <  1:129173/113‑1 (MQ=255)                                                         ncGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGAAATTTTACTGACACGGGCGAAGGGGAATTTCCTCTCCGCTCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAACCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACaa  >  1:153589/2‑248 (MQ=255)                                                                                                                                                       |                                                                                                                                                          GCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAA  >  NC_000913/13986‑14289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑